지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 1 - 12 (12page)
이용수
초록· 키워드
오류제보하기
The main functions of the epithelial sodium channel (ENaC) in the kidney distal nephron are mediation of sodium and water balance and stabilization of blood pressure. Estrogen has important effects on sodium and water balance and on premenopausal blood pressure, but its role in the regulation of ENaC function is not fully understood. Female Sprague–Dawley rats were treated with 17β-estradiol for 6 weeks following bilateral ovariectomy. Plasma estrogen, aldosterone, creatinine, and electrolytes were analyzed, and α-ENaC and derlin-1 protein expression in the kidney was determined by immunohistochemistry and western blotting. The expression levels of α-ENaC, derlin-1, AMPK, and related molecules were also examined by western blotting and real-time PCR in cultured mouse renal collecting duct (mpkCCDc14) epithelial cells following estrogen treatment. Immunofluorescence and coimmunoprecipitation were performed to detect α-ENaC binding with derlin-1 and α-ENaC ubiquitination. The results demonstrated that the loss of estrogen elevated systolic blood pressure in ovariectomized (OVX) rats. OVX rat kidneys showed increased α-ENaC expression but decreased derlin-1 expression. In contrast, estrogen treatment decreased α-ENaC expression but increased derlin-1 expression in mpkCCDc14 cells. Moreover, estrogen induced α-ENaC ubiquitination by promoting the interaction of α-ENaC with derlin-1 and evoked phosphorylation of AMPK in mpkCCDc14 cells. Our study indicates that estrogen reduces ENaC expression and blood pressure in OVX rats through derlin-1 upregulation and AMPK activation.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Extra-gonadal sites of estrogen biosynthesis and function
BMB Reports
2016 .01
-
Role of estrogen and RAS signaling in repeated implantation failure
BMB Reports
2018 .01
-
Protective role of estrogen against excessive erythrocytosis in Monge’s disease
Experimental and Molecular Medicine
2021 .01
-
A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells
조직공학과 재생의학
2021 .01
-
Exploiting the mechanism of estrogen-induced transcription to fight breast cancer
Experimental and Molecular Medicine
2021 .07
-
Tumor-Targeting Potential of Curcumin Via Estrogen Receptor In Breast Cancer
한국생물공학회 학술대회
2017 .04
-
AMPK activators: mechanisms of action and physiological activities
Experimental and Molecular Medicine
2016 .01
-
Preterm birth is associated with epigenetic programming of transgenerational hypertension in mice
Experimental and Molecular Medicine
2020 .01
-
Effects of Estrogen on Intracellular Calcium-Related T-Lymphocyte Function
조직공학과 재생의학
2016 .01
-
A novel targeting material to receptors of cancer cells
한국생물공학회 학술대회
2018 .04
-
Regulation and function of AMPK in physiology and diseases
Experimental and Molecular Medicine
2016 .01
-
Natural Product O Improve the Muscle Loss via AMPK Activation in Aged Mice
한국생물공학회 학술대회
2024 .09
-
Wnt3a-Producing Fibroblasts in Ovariectomy-Induced Osteoporosis in a Rat Model
조직공학과 재생의학
2015 .01
-
Gene Cloning, Expression, and Characterization of Anti-human Estrogen Receptor Alpha 36 Single Chain Fragment Variable
한국생물공학회 학술대회
2021 .10
-
AMPK suppresses Th2 cell responses by repressing mTORC2
Experimental and Molecular Medicine
2022 .08
-
Recombinant Expression of Anti-human Estrogen Receptor Alpha 36 Single Chain Fragment Variable
한국생물공학회 학술대회
2020 .10
-
팔뚝형 자동혈압계 평가용 혈압 시뮬레이터 개발
센서학회지
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0