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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 179 - 192 (14page)
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In this study, phytoene desaturase (PDS) in citrus and green fluorescent protein (GFP) in Nicotiana benthamiana (line 16c) were silenced by virus-induced gene silencing (VIGS) with Citrus tristeza virus (CTV), using the sense and antisense orientations. The antisense orientation showed a stronger reduction of GFP expression in N. benthamiana compared with the sense orientation. Similarly, the antisense orientation of pds induced stronger leaf photobleaching than the sense orientation. The antisense orientation produced higher levels of pds- and gfp-specific short interfering RNAs (siRNAs; ∼ 21 nucleotides) than the sense orientation. In addition, the antisense orientation of pds resulted in lower expression of carotenoid biosynthetic genes and produced lower levels of carotenoids and chlorophylls compared to the sense orientation. Furthermore, the antisense insertion resulted in higher accumulation of the colorless carotenoid phytoene compared to the sense orientation. Our results indicate that the gene fragments inserted using the antisense orientation induced higher gene silencing than the sense orientation because more short interfering RNAs were produced. These siRNAs are complementary to the mRNAs of the target gene and consequently guide the degradation of target mRNA. Better understanding of gene silencing mechanisms in plants is necessary to enhance VIGS technology.
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참고문헌 (80)
참고문헌 신청
Agüero J / 2014 / Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants / Virology 460?461:154~164
Al-Babili S / 1996 / A novel, soluble form of phytoene desaturase from Narcissus pseudonarcissus chromoplasts is Hsp70-complexed and competent for flavinylation, membrane association and enzymatic activation / Plant J 9:601~612
Albiach-Marti MR / 2004 / Citrus tristeza virus replicates and forms infectious virions in protoplasts of resistant citrus relatives / Mol Breed 14:117~128
Aliyari R / 2009 / RNA-based viral immunity initiated by the Dicer family of host immune receptors / Immunol Rev 227:176~188
Alquézar B / 2009 / Molecular and functional characterization of a novel chromoplast-specific lycopene β-cyclase from Citrus and its relation to lycopene accumulation / J Exp Bot 60:1783~1797
Al-Babili S / 1996 / A novel, soluble form of phytoene desaturase from Narcissus pseudonarcissus chromoplasts is Hsp70-complexed and competent for flavinylation, membrane association and enzymatic activation / Plant J 9:601~612
Albiach-Marti MR / 2004 / Citrus tristeza virus replicates and forms infectious virions in protoplasts of resistant citrus relatives / Mol Breed 14:117~128
Aliyari R / 2009 / RNA-based viral immunity initiated by the Dicer family of host immune receptors / Immunol Rev 227:176~188
Alquézar B / 2009 / Molecular and functional characterization of a novel chromoplast-specific lycopene β-cyclase from Citrus and its relation to lycopene accumulation / J Exp Bot 60:1783~1797
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