지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Background: The macrophages activated by lipopolisaccharide produce numerous molecules and proteins, such as tumor necrotic factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and free radicals, associated with inflammation. The response was occurred by intracellular cascaded, NF-κB pathway. So, the regulation of this pathway is very important in control of inflammation.
Objective: In this study, the anti-inflammatory and anti-oxidant effects of Sophora flavescens that is used empirically in oriental medicine and folk remedy were evaluated and the mechanism of the effects was studied.
Methods: By using the root extracts of Sophora flavescens, we performed experiment in LPS and interferon-γ (IFN-γ)-activated Raw 264.7 cells. We measured the production of NO, PGE2 and expression of iNOS and COX-2 in activated Raw 264.7 cells with Sophora flavescens root extract. Also,we tested anti-oxidant effect of Sophora flavescens root extracts by ELISA kit in activated Raw 264.7cells, and the free radical scavenging effect of material itself by DPPH assay.
Results: The Sophora flavescens root extracts decreased the production of NO (p<0.001) and PGE2(p<0.01) in Raw 264.7 cells activated by LPS and IFN-γ. The expression of proteins, iNOS and COX-2,suppressed along with the elevated concentration of Sophora flavescens root extracts. The result of DPPH assay was that the test material itself had scavenging effect for free radical (p<0.001). And the antioxidant activity in activated Raw 264.7 cells was increased with the level of the Sophora flavescens root extracts (p<0.05).
Conclusion: The Sophora flavescens root extracts suppressed the production of NO and PGE2 through the decreased expression of iNOS and COX-2. And the Sophora flavescens root extracts had the scavenging effect about free radicals itself and increased the antioxidant activity in activated macrophages.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Chae HS / 2009 / 5-Hydroxytryptophan acts on the mitogenactivated protein kinase extracellular-signal regulated protein kinase pathway to modulate cyclooxygenase-2 and induc
Chae SW / 2004 / Antioxidant Activity of Jionoside D from Clerodendron trichotomum / Biol Pharm Bull 27 (10) : 1504 ~ 1508
Hong MH / 2009 / Sophora flavescens Aiton inhibits the production of pro-inflammatory cytokines through inhibition of the NF kappaB/IkappaB signal pathway in human mast cell
Jin HJ / 2010 / Anti-inflammatory and anti-arthritic activity of the roots of Sophora flavescens / J Ethnopharmacol 127 (3) : 589 ~ 595
Kim JY / 2004 / Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages / FEBS Lett 569 (1) : 321 ~ 326
Chae SW / 2004 / Antioxidant Activity of Jionoside D from Clerodendron trichotomum / Biol Pharm Bull 27 (10) : 1504 ~ 1508
Hong MH / 2009 / Sophora flavescens Aiton inhibits the production of pro-inflammatory cytokines through inhibition of the NF kappaB/IkappaB signal pathway in human mast cell
Jin HJ / 2010 / Anti-inflammatory and anti-arthritic activity of the roots of Sophora flavescens / J Ethnopharmacol 127 (3) : 589 ~ 595
Kim JY / 2004 / Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages / FEBS Lett 569 (1) : 321 ~ 326
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Antibacterial Activity of Sophoraflavanone G Isolated from the Roots of Sophora flavescens and Red Ginseng Extract
한국미생물학회 학술대회논문집
2013 .05
-
피부 여드름 치료제 개발을 위한 천연물의 항균활성 검색
한국미생물·생명공학회지
2006 .01
-
괴화의 염색성에 관한 연구
복식
2002 .05
-
Antibacterial Activity of Sophoraflavanone G Isolated from the Roots of Sophora flavescens
Journal of Microbiology and Biotechnology
2007 .01
-
Development of simultaneous analytical method for six active compounds in sophorae radix using HPLC
한국분석과학회 학술대회
2016 .11
-
Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells
Experimental and Molecular Medicine
2009 .01
-
RAW 264.7세포에서 lipopolysaccharide로 유발시킨 염증반응에 대한 Bulnesia sarmienti 열수추출물의 억제효과
생명과학회지
2009 .04
-
보리싹 추출물의 항산화 및 항염증 활성
생명과학회지
2016 .05
-
미세캡슐화한 녹차 및 자몽종자 추출물이 Murine RAW 264.7 대식세포주의 항염증에 미치는 영향 비교
한국영양학회지
2012 .10
-
RAW 246.7 대식세포 모델에서 고삼, 감초, 백선피 에탄올 추출물 및 추출복합물의 항염증 효능 비교
생명과학회지
2014 .03
-
LPS로 유도된 RAW 264.7세포에 대한 벼메뚜기(Oxya chinensis sinuosa) 에탄올 추출물의 항염증 효과
생명과학회지
2014 .04
-
찔레나무뿌리(Rosa multiflora root)의 항산화 및 항염증효과
생명과학회지
2011 .08
-
RAW 264.7 세포에서의 단풍잎돼지풀 추출물의 항염증 활성 검증
한국미생물·생명공학회지
2020 .01
-
Antiinflammatory Effects of New Chemical Compounds, HS-1580 Series(HS-1580, HS-1581, HS-1582)
생명과학회지
2006 .12
-
Effects of Astaxanthin on the Production of NO and the Expression of COX-2 and iNOS in LPS-Stimulated BV2 Microglial Cells
Journal of Microbiology and Biotechnology
2008 .01
-
Anti-inflammatory effects of proanthocyanidin-rich red rice extract via suppression of MAPK, AP-1 and NF-κB pathways in Raw 264.7 macrophages
Nutrition Research and Practice
2016 .06
-
Anti-inflammatory effects of proanthocyanidin-rich red rice extract via suppression of MAPK, AP-1 and NF-κB pathways in Raw 264.7 macrophages
Nutrition Research and Practice
2016 .06
-
Application of response surface methodology to optimize deep eutectic solvent-based extraction of flavonoids from Flos sophorae
한국분석과학회 학술대회
2014 .06
-
Anti-inflammatory effect of the water fraction from hawthorn fruit on LPS-stimulated RAW 264.7 cells
Nutrition Research and Practice
2011 .04
-
Anti-inflammatory effect of the water fraction from hawthorn fruit on LPS-stimulated RAW 264.7 cells
Nutrition Research and Practice
2011 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0