지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Background: There are several media for culture of Malassezia spp., such as Leeming & Notman(LN) medium, modified Leeming & Notman (mLN) medium, Dixon's medium and modified Dixon'smedium etc. It is known that Malassezia spp. grow well in these media in general, but the kind andamounts of their ingredients are various and un-uniform according to researchers. Author propose thenew and transparent BHI based medium for the optimal growth of Malassezia spp.
Objectives: The purpose of this study was to design the simple and transparent BHI based mediumand find essential ingredients for the growth of M. globosa and M. obtusa.
Methods: The colony size of eight standard strains (M. dermatis, M. furfur, M. globosa, M. japonica,M. obtusa, M. sloofiae, M. sympodialis, M. yamatoensis) on the modified BHI (mBHI) agar media withdifferent ingredients was observed by naked eye after seven day culture. The compositions of mBHImedium were as follows; mBHI-1 was supplemented with 0.7% dextrose, 1.5% Tween 80, 1% glycerolto BHI medium, mBHI-2 was supplemented with 1.5% Tween 40 to mBHI-1 instead of Tween 80,mBHI-3 was supplemented with 1.5% Tween 60 to mBHI-1 instead of Tween 80, mBHI-4 was addedwith 0.8% bile salts to mBHI-1. mBHI-5 was supplemented with 1.5% Tween 60 to mBHI-4 instead ofTween 80, and mBHI-6 was supplemented with 1.5% Tween 40 to mBHI-4 instead of Tween 80. pH ofsix mBHI media was all adjusted to 6.5.
Results: M. furfur & M. japonica were grown well on mBHI-1 agar, but M. globosa & M. obtusawere not grown and others grown poorly. M. globosa & M. obtusa were not grown on mBHI-1 &mBHI-4 containing Tween 80 as lipid source, but others grown on all mBHI media. The media that alleight Malassezia strains grew well were slightly turbid mBHI-5 & transparent mBHI-6 medium.
Conclusions: M. globosa & M. obtusa need glycerol and bile salts as well as Tween 60 or 40 insteadof Tween 80 for growth. M. furfur & M japonica need not bile salts for growth. Author proposes the transparent modified BHI medium supplemented with 0.7% dextrose, 1.5% Tween 40, 1% glycerol and0.8% bile salts (mBHI-6) as new standard medium for culture of eight Malassezia species.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Cabanes FJ / 2007 / Two new lipid-dependent Malassezia species from domestic animals / FEMS Yeast Res 7 : 1064 ~ 1076
Cabanes FJ / 2011 / Malassezia cuniculi sp. nov., a novel yeast species isolated from rabbit skin / Med Mycol 49 : 40 ~ 48
Gueho E / 1996 / The genus Malassezia with description of four new species / Antonie Van Leeuwenhoek 69 : 337 ~ 355
Hirai A / 2004 / Malassezia nana sp. nov., a novel lipid-dependent yeast species isolated from animals / Int J Syst Evol Microbiol 54 : 623 ~ 627
Kaur M / 2013 / Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tertiary Care Hospital, Punjab / Indian J M
Cabanes FJ / 2011 / Malassezia cuniculi sp. nov., a novel yeast species isolated from rabbit skin / Med Mycol 49 : 40 ~ 48
Gueho E / 1996 / The genus Malassezia with description of four new species / Antonie Van Leeuwenhoek 69 : 337 ~ 355
Hirai A / 2004 / Malassezia nana sp. nov., a novel lipid-dependent yeast species isolated from animals / Int J Syst Evol Microbiol 54 : 623 ~ 627
Kaur M / 2013 / Study of the distribution of Malassezia species in patients with pityriasis versicolor and healthy individuals in Tertiary Care Hospital, Punjab / Indian J M
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