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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제5호
- 발행연도
- 2018.1
- 수록면
- 718 - 731 (14page)
이용수
초록· 키워드
오류제보하기
The beneficial effects of lactic acid bacteria (LAB) have been intensively investigated in recent decades with special focus on modulation of the host intestinal microbiota. Numerous discoveries of effective probiotics are driven by a significantly increasing demand for dietary supplements. Consequently, technological advances in the large-scale production and lyophilization are needed by probiotic-related industries for producing probiotic LAB for commercial use. Our study had a dual objective, to determine the optimum growth medium composition and to investigate appropriate cryoprotective additives (CPAs) for Lactobacillus salivarius, and compare its responses with other Lactobacillus species. The one-factor-at-a-time method and central composite design were applied to determine the optimal medium composition for L. salivarius cultivation. The following composition of the medium was established (per liter): 21.64 g maltose, 85 g yeast extract, 1.21 ml Tween 80, 6 g sodium acetate, 0.2 g MgSO4∙7H2O, 0.02 g MnSO4∙H2O, 1 g K2HPO4, 1.5 g KH2PO4, 0.01 g FeSO4∙7H2O, and 1 g sodium citrate. A cryoprotective additive combination comprising 10% (w/v) skim milk and 10% (w/v) sucrose supplemented with 2.5% (w/v) sodium glutamate was selected for L. salivarius, and its effectiveness was confirmed using culture-independent methods in the freeze-dried cells of the Lactobacillus strains. In conclusion, the optimized medium enhanced the species-specific cultivation of L. salivarius. On the other hand, the cryoprotective effects of the selected CPA mixture may also be dependent on the bacterial strain. This study highlights the necessity for precise and advanced processing techniques for large-scale production of probiotics in the food and feed industries.
목차
등록된 정보가 없습니다.
참고문헌 (65)
참고문헌 신청
Ljungh A / 2006 / Lactic acid bacteria as probiotics / Curr. Issues Intest. Microbiol. 7:73~89
Holzapfel WH / 2014 / Introduction to the LAB;Lactic Acid Bacteria – Biodiversity and Taxonomy / John Wiley & Sons Ltd. :1~12
Serban DE / 2014 / Gastrointestinal cancers: influence of gut microbiota, probiotics and prebiotics / Cancer Lett. 345:258~270
Dahiya DK / 2017 / Gut microbiota modulation and its relationship with obesity using prebiotic fibers and probiotics: a review / Front. Microbiol. 8:563
Sanchez B / 2017 / Probiotics, gut microbiota, and their influence on host health and disease / Mol. Nutr. Food Res. 61:1600240
Holzapfel WH / 2014 / Introduction to the LAB;Lactic Acid Bacteria – Biodiversity and Taxonomy / John Wiley & Sons Ltd. :1~12
Serban DE / 2014 / Gastrointestinal cancers: influence of gut microbiota, probiotics and prebiotics / Cancer Lett. 345:258~270
Dahiya DK / 2017 / Gut microbiota modulation and its relationship with obesity using prebiotic fibers and probiotics: a review / Front. Microbiol. 8:563
Sanchez B / 2017 / Probiotics, gut microbiota, and their influence on host health and disease / Mol. Nutr. Food Res. 61:1600240
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