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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 1 - 7 (7page)
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Background: The physiologic characteristics of the cashmere trait and many of the differentially expressed genes relevant to hair cycling have been extensively studied, whereas genes involved in the prenatal development of hair follicles have been poorly investigated in cashmere goats. The aim of this study, therefore, was to quantify the time-course changes in the expressions of TRα and CRABPII genes in the fetal skin of Chinese cashmere goats at the multiple embryonic days (E70, E75, E80, E90, E100, E120 and E130) using real-time quantitative PCR (RT-qPCR).
Results: RT-qPCR showed that TRα was expressed at E70 with relatively high level and then slightly decreased (E75, E80, and E90). The highest expression of TRα mRNA was revealed at E130 (P > 0.05). The expression pattern of CRABPII mRNA showed an ‘up-down-up’ trend, which revealed a significantly highest expression at E75 (P < 0.05) and was down-regulated during E80 to E120 (P < 0.05) and mildly increased at E130, subsequently.
Conclusion: This study demonstrated that TRα and CRABPII genes expressed in different levels during prenatal development of cashmere. The present study will be helpful to provide the comprehensive understanding of TRα and CRABPII genes expressions during cashmere formation and lay the ground for further studies on their roles in regulation of cashmere growth in goats.
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참고문헌 (21)
참고문헌 신청
B. Thiyagarajan / 2009 / Ameliorating effect of vitamin E on in vitro development of preimplantation buffalo embryos / Journal of Assisted Reproduction and Genetics 26 (4) :
Carabatsos MJ / 2000 / Oocyte-granulosa cell heterologous gap junctions are required for the coordination of nuclear and cytoplasmic meiotic competence / Dev Biol 226 (2) : 1
Chow CK / 1979 / Nutritional influence on cellular antioxidant defense systems / Am J Clin Nutr 32 (5) : 1066 ~ 1081
Critser ES / 1986 / Use of a fluorescent stain for visualization of nuclear material in living oocytes and early embryos / Stain Technol 61 (1) : 1 ~ 5
Dalvit G / 2005 / Effect of alpha-tocopherol and ascorbic acid on bovine oocyte in vitro maturation / Reprod Domest Anim 40 (2) : 93 ~ 97
Carabatsos MJ / 2000 / Oocyte-granulosa cell heterologous gap junctions are required for the coordination of nuclear and cytoplasmic meiotic competence / Dev Biol 226 (2) : 1
Chow CK / 1979 / Nutritional influence on cellular antioxidant defense systems / Am J Clin Nutr 32 (5) : 1066 ~ 1081
Critser ES / 1986 / Use of a fluorescent stain for visualization of nuclear material in living oocytes and early embryos / Stain Technol 61 (1) : 1 ~ 5
Dalvit G / 2005 / Effect of alpha-tocopherol and ascorbic acid on bovine oocyte in vitro maturation / Reprod Domest Anim 40 (2) : 93 ~ 97
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