지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제10권 제1호
- 발행연도
- 2005.1
- 수록면
- 13 - 17 (5page)
이용수
초록· 키워드
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In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene,ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B (43% protein sequence identity), followed by Locusta migratoria-76 (42% protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of
larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirusinfected insect cells.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
A / 1986 / Developmental expression of three genes for larvalcuticular proteins of the tobacco hornworm : 82 ~ 94
Andersen / 1993 / Primary structure of a 14 kDa basic structural protein from the cuticle of the migratory locust : 391 ~ 76 402
Andersen / 1997 / Sequencestudies of proteins from larval and pupal cuticle of the yel-low meal worm : 121 ~ 131
Andersen, S. O / 1995 / Insectcuticular proteins 25 : 153 ~ 176
Binger / 1994 / Identification of thecDNA gene and promoter for a major protein from flexiblecuticles of the giant silkmoth Hyalophora cecropia : 989 ~ 1000
Andersen / 1993 / Primary structure of a 14 kDa basic structural protein from the cuticle of the migratory locust : 391 ~ 76 402
Andersen / 1997 / Sequencestudies of proteins from larval and pupal cuticle of the yel-low meal worm : 121 ~ 131
Andersen, S. O / 1995 / Insectcuticular proteins 25 : 153 ~ 176
Binger / 1994 / Identification of thecDNA gene and promoter for a major protein from flexiblecuticles of the giant silkmoth Hyalophora cecropia : 989 ~ 1000
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