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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제22권 제2호
- 발행연도
- 2011.1
- 수록면
- 59 - 64 (6page)
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Protein kinase C (PKC) is involved in many cellular signaling pathways, it participates in many physiological processes, such as cell cycle, growth, proliferation,differentiation and apoptosis. To investigate the effect of PKC on the silkworm midgut tissue infection of Bombyx mori parvo-like virus (BmPLV), a B. mori atypical protein kinase C (BmaPKC) gene was cloned from larval midgut tissue, expressed in E. coli and purified. Additionally, the BmPLV susceptible silkworm strain and resistant silkworm strain were used to test the effect of the B. mori infection on BmPLV.
The result showed that BmaPKC encodes a predicted 586 amino acid protein, which contains a C-terminal kinase domain and an N-terminal regulatory domain.
The maximum expression amount of the soluble (His)6-tagged fusion protein was detected after 0.8mmol/L IPTG was added and cultured at 21oC. The (His) 6-tagged fusion protein revealed about 73 kDa molecular weight which confirmed by western blot and mass spectrography. Furthermore BmaPKC protein were detected at 0-72 h post-infection in BmPLVinfected larval midgut tissue, western blot showed that as time went on, the expression of BmaPKC increased gradually in susceptible strain, the expression quantity on 72 h is 5 times of 0 h. However, in resistant strain,the expression quantity is slightly lower than susceptible strain. But no significant change in resistant strain was observed as time went on. The available data suggest that BmaPKC may involve in the regulation of BmPLV proliferation.
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참고문헌 (14)
참고문헌 신청
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 2
Dedos SG / 2008 / Protein kinase A and C are ‘‘Gatekeepers’’ of capacitative Ca2+ entry in the prothoracic gland cells of the silkworm, Bombyx mori / J Insect Physiol 54
Dettwiler S / 1999 / DNA unwinding functions of minute virus of mice NS1 protein are modulated specifically by the lambda isoform of protein kinase C / J Virol 73 : 7410 ~ 74
Kim S / 2009 / Kinase-activity-independent functions of atypical protein kinase C in Drosophila / J Cell Sci 122 : 3759 ~ 3771
Kovac J / 2007 / Expression of the atypical protein kinase C (aPKC) isoforms [iota]/[lambda] and [zeta] during mouse embryogenesis / Gene Exp Patt 7 : 187 ~ 196
Dedos SG / 2008 / Protein kinase A and C are ‘‘Gatekeepers’’ of capacitative Ca2+ entry in the prothoracic gland cells of the silkworm, Bombyx mori / J Insect Physiol 54
Dettwiler S / 1999 / DNA unwinding functions of minute virus of mice NS1 protein are modulated specifically by the lambda isoform of protein kinase C / J Virol 73 : 7410 ~ 74
Kim S / 2009 / Kinase-activity-independent functions of atypical protein kinase C in Drosophila / J Cell Sci 122 : 3759 ~ 3771
Kovac J / 2007 / Expression of the atypical protein kinase C (aPKC) isoforms [iota]/[lambda] and [zeta] during mouse embryogenesis / Gene Exp Patt 7 : 187 ~ 196
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