지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제17권 제2호
- 발행연도
- 2008.1
- 수록면
- 223 - 228 (6page)
이용수
초록· 키워드
오류제보하기
A novel beetle antimicrobial protein from stimulated Copris tripartitus and the corresponding gene were isolated in parallel through differential display-PCR and expression in Escherichia coli. To find cDNA clones responsible for bacteria resistance, the suppression subtractive hybridization and GeneFishing differentially expressed genes system were employed in the dung beetle, Copris tripartitus immunized with lipopolysaccaride.
One cDNA clone from eight subtracted clones was selected through dot blot analysis and confirmed by northern blot analysis. The 516-bp, selected cDNA clone was determined by 5’ and 3’ rapid amplication of cDNA ends and cloned into the GST fusion expression vector pGEX-4T-1 for expression of the protein. The expressed protein was predicted 14.7 kDa and inhibited the growth of gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa.
These results implied that the expressed protein is related to immune defense mechanism against microorganism.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Almeida, M.S. / 2001 / cDNA cloning and heterologous expression of functional cystein-rich antifungal protein Psd1 in the yeast Pichia pastoris / Arch Biochem Biophys 395 : 1
Bang, H. S. / 2003 / Development of Copris tripartitus (Coleoptera: Scarabaeidae) in two different rearing media / Korean J. Entomol 33 : 201 ~ 204
Boman, H. G. / 1995 / Peptide antibiotics and their role in innate immunity / Annu. Rev. Immunol 13 : 61 ~ 92
Bulet, P. / 2004 / Anti-microbial peptides: from invertebrates to vertebrates / Immunol. Rev 198 : 169 ~ 184
De Lucca, A. J. / 1999 / Antifungal peptides: novel therapeutic compounds against emerging pathogens / Antimicrob. Agents Cemother 43 : 1 ~ 11
Bang, H. S. / 2003 / Development of Copris tripartitus (Coleoptera: Scarabaeidae) in two different rearing media / Korean J. Entomol 33 : 201 ~ 204
Boman, H. G. / 1995 / Peptide antibiotics and their role in innate immunity / Annu. Rev. Immunol 13 : 61 ~ 92
Bulet, P. / 2004 / Anti-microbial peptides: from invertebrates to vertebrates / Immunol. Rev 198 : 169 ~ 184
De Lucca, A. J. / 1999 / Antifungal peptides: novel therapeutic compounds against emerging pathogens / Antimicrob. Agents Cemother 43 : 1 ~ 11
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Proteomic Assessment of Dung Beetle, Copris tripartitus Immune Response
International Journal of Industrial Entomology
2008 .01
-
Additional mitochondrial DNA sequences from the dung beetle, Copris tripartitus (Coleoptera: Scarabaeidae), an endangered species in South Korea
International Journal of Industrial Entomology
2018 .01
-
Apriona germari Larval Cuticle Protein Genes: Genomic Structure of Three Cuticle Protein Genes and cDNA Cloning of a Novel Cuticle Protein
International Journal of Industrial Entomology
2007 .01
-
A LIM Protein Gene Homologue of Protaetia brevitarsis: cDNA Cloning and mRNA Expression
International Journal of Industrial Entomology
2005 .01
-
기술적 측면에서의 인간복제-그에 따른 신학적 인간이해와 기독교 교육의 방향-
기독교교육정보
2001 .04
-
Molecular Cloning of a LIM Protein cDNA from the Mulberry Longicorn Beetle, Apriona germari
International Journal of Industrial Entomology
2004 .01
-
Characterization of Expressed Sequence Tags (ESTs) Generated from the Bombyx mandarina Whole Larvae and Molecular Cloning of Serine Protease Homologue Gene
International Journal of Industrial Entomology
2004 .01
-
Comparative Study of Anti-Apoptotic Genes, Bcl-2 and P35 for the Suppression of Apoptosis Induced in Suspension Culture of Transformed Trichoplusia ni BTI Tn 5B1-4 Cells
International Journal of Industrial Entomology
2004 .01
-
생물학적 통제의 시대 인간복제(human cloning)에 대한 윤리적 논의: 불교와 기독교 시각을 중심으로
영어권문화연구
2015 .01
-
cDNA Cloning and mRNA Expression of a Cuticle Protein Gene Homologue from Protaetia brevitarsis
International Journal of Industrial Entomology
2005 .01
-
Construction of the Full-length cDNA Library and Selection of Diapause -Associated cDNA Clones from Bombyx mori Diapausing Eggs and Diapause-Activated Eggs
International Journal of Industrial Entomology
2004 .01
-
Cloning and mRNA Expression of an Actin cDNA from the Mulberry Longicorn Beetle, Apriona germari
International Journal of Industrial Entomology
2004 .01
-
Molecular Cloning and Characterization of a Novel Cuticle Protein Gene from the Chinese Oak Silkmoth, Antheraea pernyi
International Journal of Industrial Entomology
2005 .01
-
Bombyx mori b-tubulin Promoter for High-level Expression of Heterologous Genes
International Journal of Industrial Entomology
2019 .01
-
인간복제에 관한 윤리적 고찰
통일인문학
2003 .06
-
Molecular Cloning of the Myosin Light Chain-2 cDNA of Gryllotalpa orientalis
International Journal of Industrial Entomology
2004 .01
-
[특집 - 과학기술학(STS)자들이 '황우석 사건'을 본격 분석하다] 동물복제에서 인간배아복제로 - 황우석 연구팀의 복제기술 진화
역사비평
2006 .02
-
A Chymotrypsin Gene Homologue from the Mulberry Longicorn Beetle, Apriona germari: cDNA Sequence Characterization and mRNA Expression Pattern
International Journal of Industrial Entomology
2005 .01
-
Molecular Cloning and mRNA Expression a Glutathione S-Transferase cDNA from the Spider, Araneus ventricosus
International Journal of Industrial Entomology
2004 .01
-
Identification of a novel immune-related gene in the immunized black soldier fly, Hermetia illucens (L.)
International Journal of Industrial Entomology
2018 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0