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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 39 - 44 (6page)
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초록· 키워드
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Purpose: To investigate the effect of insulin on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells and the enzymatic synthetic pathway of tetrahydrobiopterin (BH4) synthesis.
Methods: Primarily cultured human TM cells were exposed to 1, 10, and 100 µg/ml of insulin and 0, 1, 10, 100 and 1000 nM dexamethasone for 3 days. To evaluate the enzymatic pathway of BH4 synthesis, 10 µM dexamethasone, 5 mM diaminopyrimidinone, 100 µM ascorbic acid, 100 µM sepiapterin, or 10 µM methotrexate were also co-administered respectively. Cellular survival and NO production were measured with MTT and Griess assay.
Results: Insulin enhanced NO production in a dose-dependent manner significantly (p<0.05) without affecting cell viability, whereas dexamethasone inhibited NO production. With co-exposure of insulin, diaminopyrimidinone and sepiapterin inhibited insulin-induced NO production. Ascorbic acid increased NO production independent of insulin and methotrexate did not affect to the action of insulin in NO production.
Conclusions: Insulin increases NO production in TM cells via de novo synthetic pathway for BH4 synthesis. Insulin could be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.
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참고문헌 (29)
참고문헌 신청
Becker B / 1990 / Topical 8-bromo-cyclic GMP lowers intraocular pressure 31 : 1647 ~ 9
Becquet F / 1997 / Nitric oxide in the eye:multifaceted roles and diverse outcomes 42 : 71 ~ 82
Bredt DS / 1994 / Nitric oxide:a physiologic messenger molecule 63 : 175 ~ 95
Brodsky SV / 2001 / Glucose scavenging of nitric oxide 280 : F480 ~ F486
Ding Y / 2000 / Effects of simulated hyperglycemia, insulin, and glucagons on endothelial nitric oxide synthase expression 279 : E11 ~ E17
Becquet F / 1997 / Nitric oxide in the eye:multifaceted roles and diverse outcomes 42 : 71 ~ 82
Bredt DS / 1994 / Nitric oxide:a physiologic messenger molecule 63 : 175 ~ 95
Brodsky SV / 2001 / Glucose scavenging of nitric oxide 280 : F480 ~ F486
Ding Y / 2000 / Effects of simulated hyperglycemia, insulin, and glucagons on endothelial nitric oxide synthase expression 279 : E11 ~ E17
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