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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 445 - 454 (10page)
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초록· 키워드
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Background: Phosphatidic acid (PA), an important second messenger, is involved in inflammation. Notably, cell-cell interactions via adhesion molecules play a central role in inflammation. This thesis show that PA induces expression of intercellular adhesion molecule-1 (ICAM-1) on macrophages and describe the signaling pathways.
Materials and methods: Macrophages were cultured in the presence of 10% FBS and assayed cell to cell adhesion using HUVEC. For the gene and protein analysis, RT-PCR, Western blot and flow cytometry were performed. In addition, overexpressed cell lines for dominant negative PKC-δ mutant established and tested their effect on the promoter activity and expression of ICAM-1 protein by PA.
Results: PA-activated macrophages significantly increased adhering to human umbilical vein endothelial cell and this adhesion was mediated by ICAM-1. Pretreatment with rottlerin (PKC-δinhibitor) or expression of a dominant negative PKC-δmutant, but not Go6976 (classical PKC-α inhibitor) and myristoylated PKC-ζinhibitor, attenuated PA-induced ICAM-1 expression. The p38 mitogen-activated protein kinase (MAPK) inhibitor blocked PA-induced ICAM-1 expression in contrast, ERK upstream inhibitor didn’t block ICAM-1.
Conclusion: These data suggest that PA-induced ICAM-1 expression and cell-cell adhesion in macrophages requires PKC-δactivation and that PKC-δactivation is triggers to sequential activation of p38 MAPK.
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참고문헌 (21)
참고문헌 신청
/ / Phosphatidic acid activation of protein kinase C-zeta overexpressedin COS cells comparison with other protein kinase C isotypes and other acidic lipids : 1001 ~ 3 1008
/ 2000 / Dynamin is membrane-active: lipid insertion is induced by phosphoinositides and phosphatidic acid 39 (40) : 12485 ~ 12493
/ 2000 / The recruitment of Raf-1 to membranes is mediated by direct interaction with phosphatidic acid and is independent of association with Ras 275 (31) : 23911 ~ 23918
/ 2001 / Differential binding of traffic-related proteins to phosphatidic acid- or phosphatidylinositol (4,5)-bisphosphate-coupled affinity reagents 276 (12) : 8987 ~ 8994
/ 2001 / IL-12 inhibition of endothelial cell functions and angiogenesis depends on lymphocyte-endothelial cell cross-talk 166 (6) : 3890 ~ 3899
/ 2000 / Dynamin is membrane-active: lipid insertion is induced by phosphoinositides and phosphatidic acid 39 (40) : 12485 ~ 12493
/ 2000 / The recruitment of Raf-1 to membranes is mediated by direct interaction with phosphatidic acid and is independent of association with Ras 275 (31) : 23911 ~ 23918
/ 2001 / Differential binding of traffic-related proteins to phosphatidic acid- or phosphatidylinositol (4,5)-bisphosphate-coupled affinity reagents 276 (12) : 8987 ~ 8994
/ 2001 / IL-12 inhibition of endothelial cell functions and angiogenesis depends on lymphocyte-endothelial cell cross-talk 166 (6) : 3890 ~ 3899
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