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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제5호
- 발행연도
- 2015.1
- 수록면
- 672 - 680 (9page)
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As a global regulatory gene in Streptomyces, afsR can activate the biosynthesis of many secondary metabolites. The effect of afsR on the biosynthesis of a phenazine metabolite, lomofungin, was studied in Streptomyces lomondensis S015. There was a 2.5-fold increase of lomofungin production in the afsR-overexpressing strain of S. lomondensis S015 N1 compared with the wild-type strain. Meanwhile, the transcription levels of afsR and two important genes involved in the biosynthesis of lomofungin (i.e., phzC and phzE) were significantly upregulated in S. lomondensis S015 N1. The optimization of metal chlorides was investigated to further increase the production of lomofungin in the afsR-overexpressing strain. The addition of different metal chlorides to S. lomondensis S015 N1 cultivations showed that CaCl2, FeCl2, and MnCl2 led to an increase in lomofungin biosynthesis. The optimum concentrations of these metal chlorides were obtained using response surface methodology. CaCl2 (0.04 mM), FeCl2 (0.33 mM), and MnCl2 (0.38 mM) gave a maximum lomofungin production titer of 318.0 ± 10.7 mg/l, which was a 4.1-fold increase compared with that of S. lomondensis S015 N1 without the addition of a metal chloride. This work demonstrates that the biosynthesis of phenazine metabolites can be induced by afsR. The results also indicate that metal chlorides addition might be a simple and useful strategy for improving the production of other phenazine metabolites in Streptomyces.
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참고문헌 (24)
참고문헌 신청
Beifuss U / 2005 / Methanophenazine and other natural biologically active phenazines / Top. Curr. Chem 244 : 77 ~ 113
Bergy ME / 1969 / Lomofungin, a new broad-spectrum antibiotic:isolation and characterization / J. Antibiot 22 : 126 ~ 128
Bierman M / 1992 / Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp / Gene 116 : 43 ~ 49
Cardone MH / 2012 / Preparation of biphenyl or biphenazine compounds useful for treating hematopoietic cancers / US Patent 20120225851
Chen L / 2012 / Highthroughput screening for Streptomyces antibiotic biosynthesis activators / Appl. Environ. Microbiol 78 : 4526 ~ 4528
Bergy ME / 1969 / Lomofungin, a new broad-spectrum antibiotic:isolation and characterization / J. Antibiot 22 : 126 ~ 128
Bierman M / 1992 / Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp / Gene 116 : 43 ~ 49
Cardone MH / 2012 / Preparation of biphenyl or biphenazine compounds useful for treating hematopoietic cancers / US Patent 20120225851
Chen L / 2012 / Highthroughput screening for Streptomyces antibiotic biosynthesis activators / Appl. Environ. Microbiol 78 : 4526 ~ 4528
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