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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제5호
- 발행연도
- 2016.1
- 수록면
- 829 - 836 (8page)
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초록· 키워드
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Penicillin G acylase (PGA) was immobilized on magnetic Fe3O4@chitosan nanoparticles through the Schiff base reaction. The immobilization conditions were optimized as follows: enzyme/support 8.8 mg/g, pH 6.0, time 40 min, and temperature 25oC. Under these conditions, a high immobilization efficiency of 75% and a protein loading of 6.2 mg/g-support were obtained. Broader working pH and higher thermostability were achieved by the immobilization. In addition, the immobilized PGA retained 75% initial activity after ten cycles. Kinetic parameters Vmax and Km of the free and immobilized PGAs were determined as 0.91 mmol/min and 0.53 mmol/min, and 0.68 mM and 1.19 mM, respectively. Synthesis of amoxicillin with the immobilized PGA was carried out in 40% ethylene glycol at 25°C and a conversion of 72% was obtained. These results showed that the immobilization of PGA onto magnetic chitosan nanoparticles is an efficient and simple way for preparation of stable PGA.
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참고문헌 (45)
참고문헌 신청
Abdulla R / 2013 / Characterization of cross linked Burkholderia cepacia lipase in alginate and κ-carrageenan hybrid matrix / J. Taiwan Inst. Chem. Eng 44:545~551
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Chao C / 2013 / Surface modification of halloysite nanotubes with dopamine for enzyme immobilization / ACS Appl. Mater. Interfaces 5:10559~10564
Deng S / 2015 / Efficient enzymatic synthesis of ampicillin by mutant Alcaligenes faecalis penicillin G acylase / J. Biotechnol 199:62~68
Guo-Li Gong / 2015 / Enhanced Production of Epothilone by Immobilized Sorangium cellulosum in Porous Ceramics / Journal of Microbiology and Biotechnology 25(10):1653~1659
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Chao C / 2013 / Surface modification of halloysite nanotubes with dopamine for enzyme immobilization / ACS Appl. Mater. Interfaces 5:10559~10564
Deng S / 2015 / Efficient enzymatic synthesis of ampicillin by mutant Alcaligenes faecalis penicillin G acylase / J. Biotechnol 199:62~68
Guo-Li Gong / 2015 / Enhanced Production of Epothilone by Immobilized Sorangium cellulosum in Porous Ceramics / Journal of Microbiology and Biotechnology 25(10):1653~1659
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