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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 245 - 253 (9page)
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초록· 키워드
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The base sequences representing human- and cow-specific 16S rRNA gene markers identified in a T-RFLP analysis were recovered from clone libraries. The human- and cowspecific primers were designed from these sequences and their specificities were analyzed with fecal DNAs from human, cow, and pig. The AllBac primer set showed positive results for all human, cow, and pig samples, whereas the human-specific primer set showed positive result only for the human sample but not for the cow or pig samples.
Likewise, the cow-specific primer set showed positive results only for the cow sample but not for the human or pig samples. Real-time PCR assay with these primers was developed for the identification and quantification of fecal pollution in the river water. The human- and cow-specific markers were detected in the order of 9 log10 copies per gram wet feces, which were two orders of magnitude lower than those of total Bacteroidales. For the river water samples, the human-specific marker was detected in 1.7-6.2 log10 copies/100 ml water, which was 2.4-4.9 orders of magnitude lower than those of total Bacteroidales. There was no significant correlation between total Bacteroidales and conventional fecal indicators, but there was a high correlation between Bacteroidales and the human-specific marker. This assay could reliably identify and quantify the fecal pollution sources, enabling effective measures in the watersheds and facilitating water quality management.
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참고문헌 (19)
참고문헌 신청
Ashelford, K. E / 2006 / New screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras / Appl. Environ. Microbiol 72 : 5734 ~ 5741
Bernhard, A. E / 2000 / A PCR assay to discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA / Appl. Enviro
Bernhard, A. E / 2000 / Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes
Carson, C. A / 2005 / Specificity of a Bacteroides thetaiotaomicron marker for human feces / Appl. Environ. Microbiol 71 : 4945 ~ 4949
Ju-Yong Jeong / 2008 / Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polym
Bernhard, A. E / 2000 / A PCR assay to discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA / Appl. Enviro
Bernhard, A. E / 2000 / Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes
Carson, C. A / 2005 / Specificity of a Bacteroides thetaiotaomicron marker for human feces / Appl. Environ. Microbiol 71 : 4945 ~ 4949
Ju-Yong Jeong / 2008 / Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polym
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