지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제2호
- 발행연도
- 2005.1
- 수록면
- 310 - 320 (11page)
이용수
초록· 키워드
오류제보하기
A membrane-based oligonucleotide array wasused to detect predominant bacterial species in human fecalsamples. Digoxygenin-labeled 16S rDNA probes were generatedby PCR from DNA that had been extracted from fecal samplesor slurries. These probes were hybridized to an array of 120oligonucleotides with sequences specific for 40 differentbacterial species commonly found in human feces, followedby color development using an alkaline phosphatase-conjugatedantibody and NBT/BCIP. Twenty of the species were detectedby this method, but E. coli, which was present at ~1×105 CFUper gram feces, was not detected. To improve the sensitivityof this assay, reverse transcriptase-PCR was used to generateprobes from RNA extracted from fecal cultures. Coupledwith a chemiluminescence detection method, this approachlowered the detection limit for E. coli from ~1×106 to ≤1×105.These results indicate that the membrane-array method withreverse transcriptase-PCR and chemiluminescence detectioncan simultaneously identify bacterial species present in fecalsamples at cell concentrations as low as ≤1×105 CFU per gram.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
A / 1995 / Design and evaluation of BacteroidesDNA probes for the specific detection of human fecalpollution 61 : 1171 ~ 1179
A / 1996 / Community analysis of thebacterial assemblages in the winter cover and pelagic layersof a high mountain lake by in situ hybridization 62 : 2138 ~ 2144
A / 1998 / Design and evaluation of a 16S rRNA-targetedoligonucleotide probe for specific detection and quantitationof human faecal Bacteroides populations 21 : 65 ~ 71
A / 2003 / Comparison of real-time PCR with SYBR Green I or 5´-nuclease assays and dot-blot hybridization with rDNAtargetedoligonucleotide probes in quantification of selec
Bae / 2003 / Transformation of ginsenosides to compoundK by lactic acid bacteria of human intestine 13 : 9 ~ 901 14
A / 1996 / Community analysis of thebacterial assemblages in the winter cover and pelagic layersof a high mountain lake by in situ hybridization 62 : 2138 ~ 2144
A / 1998 / Design and evaluation of a 16S rRNA-targetedoligonucleotide probe for specific detection and quantitationof human faecal Bacteroides populations 21 : 65 ~ 71
A / 2003 / Comparison of real-time PCR with SYBR Green I or 5´-nuclease assays and dot-blot hybridization with rDNAtargetedoligonucleotide probes in quantification of selec
Bae / 2003 / Transformation of ginsenosides to compoundK by lactic acid bacteria of human intestine 13 : 9 ~ 901 14
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Oligonucleotide Array-based Detection and Genotyping of Mollicutes (Acholeplasma, Mycoplasma, and Ureaplasma)
Journal of Microbiology and Biotechnology
2009 .01
-
PCR-Based Detection of Mycoplasma Species
The Journal of Microbiology
2006 .02
-
Multiplex PCR for Species-Specific Detection of the Opportunistic Human Fungal Pathogens
한국미생물학회 학술대회논문집
2014 .04
-
Rapid and Sensitive Detection of Listeria monocytogenes Using a PCR-Enzyme-Linked Immunosorbent Assay
Journal of Microbiology and Biotechnology
2008 .01
-
Quick Real-time PCR을 이용한 Avian Influenza Virus Subtype H5N1의 신속검출법
미생물학회지
2007 .03
-
Real-time PCR을 이용한 가축생균제용 유산균 정량분석
생명과학회지
2010 .12
-
Development of Genus- and Species-Specific Probe Design System forPathogen Detection Based on 23S rDNA
Journal of Microbiology and Biotechnology
2006 .01
-
DNA Chemiluminescence by Phenanthroline-CU(II)
한국광과학회 학술대회
1999 .01
-
PATTERNS IN IRREGULAR MULTI-DIMENSIONAL ARRAYS
Journal of applied mathematics & computing
2005 .01
-
Development of RT-PCR and Nested PCR Assay for the Detection of Non-reported Five Seed-transmitted Viruses in Quarantine
한국미생물학회 학술대회논문집
2014 .04
-
Quantitative Analysis of Human- and Cow-Specific 16S rRNA Gene Markers for Assessment of Fecal Pollution in River Waters by Real-Time PCR
Journal of Microbiology and Biotechnology
2010 .01
-
Application of LATE-PCR to Detect Candida and Aspergillus Fungal Pathogens by a DNA Hybridization Assay
한국미생물·생명공학회지
2017 .01
-
Development of TaqMan Probe-Based Real-Time PCR Method for erm(A), erm(B), and erm(C), Rapid Detection of Macrolide-Lincosamide- Streptogramin B Resistance Genes, from Clinical Isolates
Journal of Microbiology and Biotechnology
2009 .01
-
Detection of Human Adenoviruses and Enteroviruses in Korean Oysters Using Cell Culture, Integrated Cell Culture-PCR, and Direct PCR
The Journal of Microbiology
2006 .04
-
Specific PCR Detection of an Oil-Degrading Bacterium, Corynebacterium sp. IC10, Using Species-Specific Primers of 16S rDNA Sequences
한국동물학회 학술대회
1999 .01
-
Rapid Detection and Monitoring Therapeutic Efficacy of Mycobacterium tuberculosis Complex Using a Novel Real-Time Assay
Journal of Microbiology and Biotechnology
2012 .01
-
Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR
Journal of Microbiology and Biotechnology
2018 .01
-
세포배양 유래 생물의약품 중 Bovine Viral Diarrhoea Virus 검출을 위한 RT-PCR, Real-Time RT-PCR 및 RT-PCR-ELISA 기법의 검출한계와 정량범위 평가
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2003 .06
-
Application Study of PCR Additives to Improve The Split Peaks in Direct PCR
한국분석과학회 학술대회
2019 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0