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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 91 - 95 (5page)
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For the detection of the oil-degrading bacterium, Nocardia sp. H17-1, inoculated during the bioremediation of oil-contaminated soil, a species-specific primer was constructed based on the 16S rDNA sequence of this strain. Two forward primers and two reverse primers were designed and tested against both closely and distantly related bacterial strains. All the primers designed were specific to the Nocardia sp. H17-1. Particularly, primer sets NH169F-NH972R and NH575F-NH972R could be used to detect 50 fg of template DNA and 1.2?104 CFU/g of sandy soil. These two PCR primer sets successfully detected the H17-1 strain in the oil-contaminated soil samples containing heterogeneous DNA. We also conformed the primer specificity by restriction-enzyme cleavage of the PCR products and denaturing gradient gel electrophoresis.
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등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
/ 1985 / Reasons for possible failure of inoculation to enhance biodegradation : 977 ~ 983
/ 1993 / Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA : 69
/ 1994 / Mineralization performance of Rhodococcus chlorophenolicus strain PCP-1 in contaminated soil stimulating on site conditions salki (ja-salonen) : 377 ~ 385
/ 1994 / Use of molecular tools evaluate the survival of a microorganism injected into an aquifer : 1059 ~ 1067
/ 1995 / A small circle of knowledge a large circle of ignorance 103 : 121 ~ 123
/ 1993 / Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA : 69
/ 1994 / Mineralization performance of Rhodococcus chlorophenolicus strain PCP-1 in contaminated soil stimulating on site conditions salki (ja-salonen) : 377 ~ 385
/ 1994 / Use of molecular tools evaluate the survival of a microorganism injected into an aquifer : 1059 ~ 1067
/ 1995 / A small circle of knowledge a large circle of ignorance 103 : 121 ~ 123
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