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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제8호
- 발행연도
- 2015.1
- 수록면
- 1,216 - 1,226 (11page)
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초록· 키워드
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GlxR is considered as a global transcriptional regulator controlling a large number of genes having broad physiological aspects in Corynebacterium glutamicum. However, the expression profile revealing the transcriptional control of glxR has not yet been studied in detail. DNA affinity chromatography experiments revealed the binding of transcriptional regulators SucR, RamB, GlxR, and a GntR-type protein (hereafter denoted as GntR3) to the upstream region of glxR. The binding of different regulators to the glxR promoter was confirmed by EMSA experiments. The expression of glxR was analyzed in detail under various carbon sources in the wild-type and different mutant strains. The sucR and gntR3 deletion mutants showed decreased glxR promoter activities, when compared with the wild type, irrespective of the carbon sources. The promoter activity of glxR was derepressed in the ramB deletion mutant under all the tested carbon sources. These results indicate that SucR and GntR3 are acting as activators of GlxR, while RamB plays a repressor. As expected, the expression of glxR in the cyaB and glxR deletion mutants was derepressed under different media conditions, indicating that GlxR is autoregulated.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Arndt A / 2007 / The alcohol dehydrogenase gene adhA in Corynebacterium glutamicum is subject to carbon catabolite repression / J. Bacteriol 189 : 7408 ~ 7416
Arndt A / 2008 / Corynebacteria: Genomics and Molecular Biology / Caister Academic Press : 155 ~ 182
Arndt A / 2008 / Ethanol catabolism in Corynebacterium glutamicum / J. Mol. Microbiol. Biotechnol 15 : 222 ~ 233
Auchter M / 2009 / Dual transcriptional control of the acetaldehyde dehydrogenase gene ald of Corynebacterium glutamicum by RamA and RamB / J. Biotechnol 140 : 84 ~ 91
Auchter M / 2011 / Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum / J. Biotechnol 152 : 77 ~ 86
Arndt A / 2008 / Corynebacteria: Genomics and Molecular Biology / Caister Academic Press : 155 ~ 182
Arndt A / 2008 / Ethanol catabolism in Corynebacterium glutamicum / J. Mol. Microbiol. Biotechnol 15 : 222 ~ 233
Auchter M / 2009 / Dual transcriptional control of the acetaldehyde dehydrogenase gene ald of Corynebacterium glutamicum by RamA and RamB / J. Biotechnol 140 : 84 ~ 91
Auchter M / 2011 / Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum / J. Biotechnol 152 : 77 ~ 86
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