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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제1호
- 발행연도
- 2017.1
- 수록면
- 72 - 76 (5page)
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Detection of exon 19 deletion mutation in the epidermal growth factor receptor (EGFR) gene, which results in increased and sustained phosphorylation of EGFR, is important for diagnosis and treatment guidelines in non-small-cell lung cancer. Here, we have developed a simple and convenient detection system using the interaction between G-quadruplex and fluorophore thioflavin T (ThT) for discriminating EGFR exon 19 deletion mutant DNA from wild type without a label and quencher. In the presence of exon 19 deletion mutant DNA, the probe DNAs annealed to the target sequences were transformed into G-quadruplex structure. Subsequent intercalation of ThT into the G-quadruplex resulted in a light-up fluorescence signal, which reflects the amount of mutant DNA. Due to stark differences in fluorescence intensity between mutant and wild-type DNA, we suggest that the induced G-quadruplex structure in the probe DNA can report the presence of cancer-causing deletion mutant DNAs with high sensitivity.
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참고문헌 (16)
참고문헌 신청
Balasubramanian S / 2011 / Targeting Gquadruplexes in gene promoters: a novel anticancer strategy? / Nat. Rev. Drug Discov 10 : 261 ~ 275
DeSantis CE / 2014 / Cancer treatment and survivorship statistics, 2014 / CA Cancer J. Clin 64 : 252 ~ 271
Deng M / 2008 / Highly effective colorimetric a nd v isual detection o f nucleic a cids u sing a n asymmetrically split peroxidase DNAzyme / J. Am. Chem. Soc 130 : 13095 ~ 1
Ellison G / 2013 / EGFR mutation testing in lung cancer:a review of available methods and their use for analysis of tumour tissue and cytology samples / J. Clin. Pathol 66 :
Huang S-M / 1999 / Epidermal growth factor receptor inhibition in cancer therapy: biology, rationale and preliminary clinical results / Invest. New Drugs 17 : 259 ~ 269
DeSantis CE / 2014 / Cancer treatment and survivorship statistics, 2014 / CA Cancer J. Clin 64 : 252 ~ 271
Deng M / 2008 / Highly effective colorimetric a nd v isual detection o f nucleic a cids u sing a n asymmetrically split peroxidase DNAzyme / J. Am. Chem. Soc 130 : 13095 ~ 1
Ellison G / 2013 / EGFR mutation testing in lung cancer:a review of available methods and their use for analysis of tumour tissue and cytology samples / J. Clin. Pathol 66 :
Huang S-M / 1999 / Epidermal growth factor receptor inhibition in cancer therapy: biology, rationale and preliminary clinical results / Invest. New Drugs 17 : 259 ~ 269
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