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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제6호
- 발행연도
- 2008.1
- 수록면
- 1,050 - 1,058 (9page)
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초록· 키워드
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A gene encoding a dextransucrase (dsrBCB4) that synthesizes only α-1,6-linked dextran was cloned from Leuconostoc mesenteroides B-1299CB4. The coding region consisted of an open reading frame (ORF) of 4,395 bp that coded a 1,465-amino-acids protein with a molecular mass of 163,581 Da. The expressed recombinant DSRBCB4 (rDSRBCB4) synthesized oligosaccharides in the presence of maltose or isomaltose as an acceptor, plus the products included α-1,6-linked glucosyl residues in addition to the maltosyl or isomaltosyl residue. Alignments of the amino acid sequence of DSRBCB4 with glucansucrases from Streptococcus and Leuconostoc identified conserved amino acid residues in the catalytic core that are critical for enzyme activity. The mutants D530N, E568Q, and D641N displayed a 98- to 10,000-fold reduction of total enzyme activity.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Bae, J. W. / 2006 / Development of recombinant Pseudomonas putida containing homologous styrene monooxygenase genes for the production of (S)-styrene oxide. Biotechnol / Biop
Barker, P. E. / 1991 / The production of the enzyme dextransucrase using nonaerated fermentation techniques. Biotechnol / Bioeng 37 : 703 ~ 707
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 7
Brown, D. E. / 1989 / A pH controlled fedbatch process for dextransucrase production. J. Chem. Technol / Biotechnol 48 : 405 ~ 414
CHUNG, CHANG-HO / 2006 / Production of Glucooligosaccharides and Mannitol from Leuconostocmesenteroides B-742 Fermentation and its Separation from Byproducts / Journal of Mic
Barker, P. E. / 1991 / The production of the enzyme dextransucrase using nonaerated fermentation techniques. Biotechnol / Bioeng 37 : 703 ~ 707
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 7
Brown, D. E. / 1989 / A pH controlled fedbatch process for dextransucrase production. J. Chem. Technol / Biotechnol 48 : 405 ~ 414
CHUNG, CHANG-HO / 2006 / Production of Glucooligosaccharides and Mannitol from Leuconostocmesenteroides B-742 Fermentation and its Separation from Byproducts / Journal of Mic
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