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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제5호
- 발행연도
- 2004.1
- 수록면
- 1,075 - 1,080 (6page)
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Dextransucrase (DSRB742) from Leuconostocmesenteroides NRRL B-742CB is a glucosyltransferase thatcatalyzes the synthesis of dextran using sucrose, or thesynthesis of oligosaccharides when acceptor molecules, likemaltose, are present. The DSRB742 gene (dsrB742) wascloned and the properties were characterized. In order toidentify critical amino acid residues, the DSRB742 aminoacid sequence was aligned with glucosyltransferase sequences,and three amino acid residues reported as sucrose bindingamino acids in Streptococcus glucosyltransferases were selectedfor site-directed mutagenesis experiments. Asp-533, Asp-536, and His-643 were independently replaced with Ala orAsn. D533A and D536A dextransucrases showed reduceddextran synthesis activities, 2.3% and 40.8% of DSRB742dextransucrase, respectively, and D533N, D536N, H643A,and H643N dextransucrases showed complete suppression ofdextran synthesis activities altogether. Additionally, D536Ndextransucrase showed complete suppression of oligosaccharidesynthesis activities. However, modifications at Asp-533 or atHis-643 retained acceptor reaction activities in the range of8.4% to 21.3% of DSRB742 acceptor reaction activity. Thusat least two carboxyl groups of Asp-533 and Asp-536, andHis-643 as a proton donor, are essential for the catalysisprocess.
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참고문헌 (28)
참고문헌 신청
[학술지(정기간행물)] -
A / 1983 / A D-glucosylated form of dextransucrase : 257 ~ 268
Fu / 1988 / Chemical modification by diethylpyrocarbonate and dye photo-oxidation : 97 ~ 109
Jeon / 2003 / Low cariogenicityof maltosyl-erythritol, major transglycosylation product oferythritol, by Bacillus stearothermophilus maltogenic amylase 13 : 815 ~ 818
Kang / 2003 / Directed evolution of a dextransucrase for increasedconstitutive activity and the synthesis of a highly brancheddextran 26 : 167 ~ 176
A / 1983 / A D-glucosylated form of dextransucrase : 257 ~ 268
Fu / 1988 / Chemical modification by diethylpyrocarbonate and dye photo-oxidation : 97 ~ 109
Jeon / 2003 / Low cariogenicityof maltosyl-erythritol, major transglycosylation product oferythritol, by Bacillus stearothermophilus maltogenic amylase 13 : 815 ~ 818
Kang / 2003 / Directed evolution of a dextransucrase for increasedconstitutive activity and the synthesis of a highly brancheddextran 26 : 167 ~ 176
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