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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제11호
- 발행연도
- 2006.1
- 수록면
- 1,753 - 1,759 (7page)
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초록· 키워드
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Paenibacillus sp. HY-8 isolated from the digestivetracts of the longicorn beetle, Moechotypa diphysis, producedan extracellular endoxylanase with a molecular weight of20 kDa estimated by SDS-PAGE. The xylanase was purifiedto near electrophoretic homogeneity from the culture supernatantafter amonium sulfate precipitation, gel filtration, and ion-exchange chromatography. The purified xylanase exhibitedoC. The Km and Vmaxvalues were 7.2 mg/ml and 16.3 U/mg, respectively, forbirchwood xylan as the substrate. Nucleotide sequence of thePCR-cloned gene was determined to have the open readingframe encoding a polypeptide of 212 amino acids. The N-terminal amino acid sequence and the nucleotide sequenceanalyses predicted that the precursor xylanase contained asignal peptide composed of 28 amino acids and a catalyticallysequence shared extensive similarity with those of the glycosidehydrolase family 11 of xylanases from other bacteria. Thepredicted amino acid sequence contained two glutamate residues,previously identified as essential and conserved for activesites in other xylanases of the glycoside hydrolase family 11.
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참고문헌 (24)
참고문헌 신청
Bae, S. H. / 1991 / Purification and characterization of an extracellular xylanase of Bacillus stearothermophilus / Kor. J. Appl. Microbiol. Biotechnol. 19 : 592 ~ 597
Beg, Q. K / 2001 / Microbial xylanases and their industrial applications: A review / Appl. Microbiol. Biotechnol. 56 : 326 ~ 338
Biely, P / 1981 / Substrate-binding site of endo-1,4-beta-xylanase of the yeast Cryptococcus albidus / Eur. J. Biochem. 119 : 559 ~ 564
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem.
Brennan, Y / 2004 / Unusual microbial xylanases from insect guts / Appl. Environ. Microbiol. 70 : 3609 ~ 3617
Beg, Q. K / 2001 / Microbial xylanases and their industrial applications: A review / Appl. Microbiol. Biotechnol. 56 : 326 ~ 338
Biely, P / 1981 / Substrate-binding site of endo-1,4-beta-xylanase of the yeast Cryptococcus albidus / Eur. J. Biochem. 119 : 559 ~ 564
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem.
Brennan, Y / 2004 / Unusual microbial xylanases from insect guts / Appl. Environ. Microbiol. 70 : 3609 ~ 3617
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