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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제10호
- 발행연도
- 2009.1
- 수록면
- 1,092 - 1,097 (6page)
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A novel exopolysaccharide named Ebosin was produced by
Streptomyces sp. 139, with medicinal activity. Its biosynthesis
gene cluster (ste) has been previously identified. For the
functional study of the ste7 gene in Ebosin biosynthesis, it
was disrupted with a double crossover via homologous
recombination. The monosaccharide composition of EPS-
7m produced by the mutant strain Streptomyces sp. 139
(ste7-) was found altered from that of Ebosin, with fucose
decreasing remarkably. For biochemical characterization of
Ste7, the ste7 gene was cloned and expressed in Escherichia
coli BL21. With a continuous coupled spectrophotometric
assay, Ste7 was demonstrated to have the ability of
catalyzing the transfer of fucose specifically from GDP-β-
L-fucose to a fucose acceptor, the lipid carrier located in
the cytoplasmic membrane of Streptomyces sp. 139 (ste7-).
Therefore, the ste7 gene has been identified to code for a
fucosyltransferase, which plays an essential role in the
formation of repeating sugars units during Ebosin biosynthesis.
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참고문헌 (23)
참고문헌 신청
Barreras, M. / 2004 / Functional characterization of GumK, a membrane-associated beta-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide
Bierman, M. / 1992 / Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp / Gene 116 : 43 ~ 49
Bitter, T. / 1962 / A modified uronic acid carbazole reaction / Anal. Biochem 4 : 330 ~ 334
Chabot, S. / 2001 / Exopolysaccharides from Lactobacillus rhamnosus RW-9595M stimulate TNF, IL-6 and IL-12 in human and mouse cultured immunocompetent cells, and IFN-γ in mo
Denis, F. / 1992 / A versatile shuttle cosmid vector for use in Escherichia coli and actinomycetes / Gene 111 : 115 ~ 118
Bierman, M. / 1992 / Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp / Gene 116 : 43 ~ 49
Bitter, T. / 1962 / A modified uronic acid carbazole reaction / Anal. Biochem 4 : 330 ~ 334
Chabot, S. / 2001 / Exopolysaccharides from Lactobacillus rhamnosus RW-9595M stimulate TNF, IL-6 and IL-12 in human and mouse cultured immunocompetent cells, and IFN-γ in mo
Denis, F. / 1992 / A versatile shuttle cosmid vector for use in Escherichia coli and actinomycetes / Gene 111 : 115 ~ 118
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Streptomyces zaisanensis sp. nov., and Streptomyces nalaikhanensis sp. nov. Two Novel Streptomyces Species from Mongolian Soil
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