지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제1호
- 발행연도
- 2009.1
- 수록면
- 99 - 108 (10page)
이용수
초록· 키워드
오류제보하기
Strain PUPC1 produces an antifungal protease as
well as plant growth promoting enzymes such as 1-
aminocyclopropane-1-carboxylate (ACC) deaminase and
phosphatase. Morphological, cultural, and physiological
characteristics as well as 16S rRNA gene-sequence-based
phylogenetic analysis confirmed the taxonomic affiliation
of PUPC1 as Chryseobacterium aquaticum. The optimum
growth of PUPC1 was observed at pH 6.0 and 30oC, and
maximum protease production was observed in medium
B amended with 1% tryptone, 0.5% sucrose, and 0.005%
MnCl2. The protease was purified by ammonium sulfate
precipitation, Sephadex G-75 gel filtration chromatography,
and electroelution from preparative SDS-PAGE. The
protease had a molecular mass of 18.5 kDa. The optimum
pH and temperature stability of the protease were pH 5.0-
10.0 and temperature 40-70oC. Chryseobacterium aquaticum
PUPC1 and its protease showed a broad-spectrum antifungal
activity against phytopathogenic fungi. Strain PUPC1 also
exhibited plant growth promoting traits. The objective of
the present investigation was to isolate a strain for
agricultural application for plant growth promotion and
biocontrol of fungal diseases.
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참고문헌 (23)
참고문헌 신청
[학술지(정기간행물)]
AYYADURAI, N. / 2007 / Functional Characterization of Antagonistic Fluorescent Pseudomonads Associated with Rhizospheric Soil of Rice (Oryza sativa L.) / Journal of Microbiol
Ayyadurai, N. / 2006 / Isolation and characterization of a novel banana rhizosphere bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana / J. A
Bric, J. M. / 1991 / Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane / Appl. Environ. Microbiol 57 : 535 ~ 538
Cattelan, A. J. / 1999 / Screening for plant growth promoting rhizobacteria to promote early soyabean growth / Soil Sci. Soc. Am. J 63 : 1670 ~ 1680
AYYADURAI, N. / 2007 / Functional Characterization of Antagonistic Fluorescent Pseudomonads Associated with Rhizospheric Soil of Rice (Oryza sativa L.) / Journal of Microbiol
Ayyadurai, N. / 2006 / Isolation and characterization of a novel banana rhizosphere bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana / J. A
Bric, J. M. / 1991 / Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane / Appl. Environ. Microbiol 57 : 535 ~ 538
Cattelan, A. J. / 1999 / Screening for plant growth promoting rhizobacteria to promote early soyabean growth / Soil Sci. Soc. Am. J 63 : 1670 ~ 1680
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