지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제11호
- 발행연도
- 2013.1
- 수록면
- 1,554 - 1,559 (6page)
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초록· 키워드
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A protease produced by Bacillus polyfermenticus SCD was purified and characterized as a new detergent material. The protease was purified from supernatant produced by B. polyfermenticus SCD, by ammonium sulfate precipitation, ion-exchange chromatography on a DEAE-Sephadex A-50, and finally gel filtration chromatography on Sephadex G-50. The molecular mass of this enzyme was 44 kDa based on SDS-PAGE. The optimum temperature and pH were 50oC and pH 8.0. The ranges of its stability to the pH and temperature were 7.0 to 9.0 and under 40oC, respectively. The enzyme was highly stable in the presence of the surfactants like Triton X-100 (0.1%), showing a 2-fold increase in its proteolytic activity.
However, the enzyme was slightly inhibited by the chelating agent EDTA (1 mM). The enzyme has a maximum activity at 50°C and the activity can be increased by surfactants such as Triton X-100 and Tween 80.
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참고문헌 (23)
참고문헌 신청
Bhaskar N / 2007 / Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activitie
Chi Z / 2007 / Optimization of medium and cultivation conditions for alkaline protease production by the marine yeast Aureobasidium pullulans / Bioresour. Technol 98 : 534 ~
Genckal H / 2006 / Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats / Enzyme Microb. Technol 39 : 703 ~ 710
Gupta R / 2002 / Bacterial alkaline proteases; molecular approaches and industrial applications / Appl. Microbiol. Biotechnol 59 : 15 ~ 32
Gupta, Rishikesh Kumar / 2012 / Scale-Up of an Alkaline Protease from Bacillus pumilus MTCC 7514 Utilizing Fish Meal as a Sole Source of Nutrients / Journal of Microbiology a
Chi Z / 2007 / Optimization of medium and cultivation conditions for alkaline protease production by the marine yeast Aureobasidium pullulans / Bioresour. Technol 98 : 534 ~
Genckal H / 2006 / Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats / Enzyme Microb. Technol 39 : 703 ~ 710
Gupta R / 2002 / Bacterial alkaline proteases; molecular approaches and industrial applications / Appl. Microbiol. Biotechnol 59 : 15 ~ 32
Gupta, Rishikesh Kumar / 2012 / Scale-Up of an Alkaline Protease from Bacillus pumilus MTCC 7514 Utilizing Fish Meal as a Sole Source of Nutrients / Journal of Microbiology a
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