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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제5호
- 발행연도
- 2008.1
- 수록면
- 859 - 865 (7page)
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초록· 키워드
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Bacterial glycosyltransferases have drawn growing attention as economical enzymes for oligosaccharide synthesis, with their easy expression and relatively broad substrate specificity.
Here, we characterized a glycosyltransferase homolog (Fnu_GT) from a human oral pathogen, Fusobacterium nucleatum. Bioinformatic analysis showed that Fnu_GT belongs to the glycosyltransferases family II. The recombinant Fnu_GT (rFnu_GT) expressed in Escherichia coli displayed the highest glycosylation activity when UDP-galactose (Gal) was used as a donor nucleotide-sugar with heptose or N-acetylglucosamine (GlcNAc) as an acceptor sugar.
Interestingly, rFnu_GT transferred the galactose moiety of UDP-Gal to a nonreducing terminal GlcNAc attached to the trimannosyl core glycan, indicating its potential as an enzyme for human-type N-glycan synthesis.
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참고문헌 (22)
참고문헌 신청
Bachrach, G. / 2004 / Characterization of the novel Fusobacterium nucleatum plasmid pKH9 and evidence of an addiction system. Appl. Environ / Microbiol 70 : 6957 ~ 6962
Bateman, A. / 2000 / The Pfam protein families database / Nucleic Acids Res 28 : 263 ~ 266
Blixt, O. / 1999 / High-level expression of the Neisseria meningitidis lgtA gene in Escherichia coli and characterization of the encoded N-acetylglucosaminyltransferase as a
Bonofiglio, L. / 2005 / Biochemical characterization of the pneumococcal glucose 1-phosphate uridylyltransferase (GalU) essential for capsule biosynthesis. Curr / Microbiol 5
Charnock, S. J. / 1999 / Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms / Biochemistry 38 : 63
Bateman, A. / 2000 / The Pfam protein families database / Nucleic Acids Res 28 : 263 ~ 266
Blixt, O. / 1999 / High-level expression of the Neisseria meningitidis lgtA gene in Escherichia coli and characterization of the encoded N-acetylglucosaminyltransferase as a
Bonofiglio, L. / 2005 / Biochemical characterization of the pneumococcal glucose 1-phosphate uridylyltransferase (GalU) essential for capsule biosynthesis. Curr / Microbiol 5
Charnock, S. J. / 1999 / Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms / Biochemistry 38 : 63
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