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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제5호
- 발행연도
- 2008.1
- 수록면
- 926 - 932 (7page)
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초록· 키워드
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Human heavy chain (H-) and light chain (L-) ferritins
were amplified from a human cDNA library. Each ferritin
gene was inserted downstream of the T7 promoter of
bacterial expression vectors, and two types of coexpression
vectors were constructed. The expression levels of
recombinant ferritins ranged about 26-36% of whole-cell
protein. H-ferritin exhibited a lower expression ratio
compared with L-ferritin, by a coexpression system. However,
the coexpression of HL-ferritins was significantly increased
above the expression ratio of H-ferritin by cultivation
without IPTG induction overnight. Purified recombinant
H-, L-, HL-, and LH-ferritins were shown to be homoand
heteropolymeric high molecular complexes and it was
indicated that their assembled subunits would be able to
work functionally in the cell. Thus, these results indicate
an improvement in the expression strategy of H-ferritin
for heteropolymeric production and studies of ferritin
assembly in Escherichia coli.
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참고문헌 (23)
참고문헌 신청
Akhayat, O. / 1987 / A new type of prosome-like particle, composed of small cytoplasmic RNA and multimers of a 21-kDa protein, inhibits protein synthesis in vitro. Eur / Bioc
Andrews, P. / 1964 / Estimation of the molecular weights of proteins by Sephadex gel-filtration / Biochem 91 : 222 ~ 233
Beaumont, C. / 1996 / Novel properties of L-type polypeptide subunits in mouse ferritin molecules. J. Biol / Chem 271 : 7923 ~ 7926
Coux, O. / 1992 / The protein of M(r) 21,000 constituting the prosome-like particle of duck erythroblasts is homologous to apoferritin. Eur / Biochem 207 : 823 ~ 832
Ford, G. C. / 1984 / Ferritin: Design and formation of an iron-storage molecule. Philos. Trans. R. Soc. Lond. B Biol / Sci 304 : 551 ~ 565
Andrews, P. / 1964 / Estimation of the molecular weights of proteins by Sephadex gel-filtration / Biochem 91 : 222 ~ 233
Beaumont, C. / 1996 / Novel properties of L-type polypeptide subunits in mouse ferritin molecules. J. Biol / Chem 271 : 7923 ~ 7926
Coux, O. / 1992 / The protein of M(r) 21,000 constituting the prosome-like particle of duck erythroblasts is homologous to apoferritin. Eur / Biochem 207 : 823 ~ 832
Ford, G. C. / 1984 / Ferritin: Design and formation of an iron-storage molecule. Philos. Trans. R. Soc. Lond. B Biol / Sci 304 : 551 ~ 565
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