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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제2호
- 발행연도
- 2008.1
- 수록면
- 299 - 307 (9page)
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초록· 키워드
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Soybean seed ferritin is essential for human iron
supplementation and iron deficiency anemia prevention
because it contains abundant bioavailable iron and is
frequently consumed in the human diet. However, it is
poorly understood in regards its several properties, such
as iron mineralization, subunit assembly, and protein
folding. To address these issues, we decided to prepare the
soybean seed ferritin complex via a recombinant DNA
approach. In this paper, we report a rapid and simple
Escherichia coli expression system to produce the soybean
seed ferritin complex. In this system, two subunits of soybean
seed ferritin, H-2 and H-1, were encoded in a single plasmid,
and optimal expression was achieved by additionally
coexpressing a team of molecular chaperones, trigger
factor and GroEL-GroES. The His-tagged ferritin complex
was purified by Ni
2+
affinity chromatography, and an
intact ferritin complex was obtained following His-tagged
enterokinase (His-EK) digestion. The purified ferritin complex
synthesized in E. coli demonstrated some reported features
of its native counterpart from soybean seed, including an
apparent molecular weight, multimeric assembly, and
iron uptake activity. We believe that the strategy described
in this paper may be of general utility in producing other
recombinant plant ferritins built up from two types of
subunits.
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참고문헌 (24)
참고문헌 신청
Arosio, P. / 1978 / On ferritin heterogeneity. Further evidence for heteropolymers. J. Biol / Chem 253 : 4451 ~ 4458
Arosio, P. / 2002 / Ferritin, iron homeostasis, and oxidative damage. Free Radic. Biol / Med 33 : 457 ~ 463
Chasteen, N. D. / 1982 / Iron binding by horse spleen apoferritin. A vanadyl (IV) EPR spin probe study. J. Biol / Chem 257 : 7672 ~ 7677
Clerte, S. / 1999 / Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin. Acta Crystallogr. D Biol / Crystal
Davila-Hicks, P. / 2004 / Iron in ferritin or in salts (ferrous sulfate) is equally bioavailable in nonanemic women. Am. J. Clin / Nutr 80 : 936 ~ 940
Arosio, P. / 2002 / Ferritin, iron homeostasis, and oxidative damage. Free Radic. Biol / Med 33 : 457 ~ 463
Chasteen, N. D. / 1982 / Iron binding by horse spleen apoferritin. A vanadyl (IV) EPR spin probe study. J. Biol / Chem 257 : 7672 ~ 7677
Clerte, S. / 1999 / Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin. Acta Crystallogr. D Biol / Crystal
Davila-Hicks, P. / 2004 / Iron in ferritin or in salts (ferrous sulfate) is equally bioavailable in nonanemic women. Am. J. Clin / Nutr 80 : 936 ~ 940
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Improved Coexpression and Multiassembly Properties of RecombinantHuman Ferritin Subunits in Escherichia coli
Journal of Microbiology and Biotechnology
2008 .01
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훼리틴 생산 재조합 효모의 철분 결핍성 빈혈 개선 효과
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Functional Assembly of Recombinant Human Ferritin Subunits in Pichia pastoris
Journal of Microbiology and Biotechnology
2007 .01
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미국흰불나방의 Ferritin의 특성과 철 섭식에 따른 변화
한국곤충학회지
1998 .01
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곤충의 Ferritin
한국동물학회 뉴스레터
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돌돔 ferritin H 유전자의 클로닝과 발현 분석
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Purification and Glycosylation Pattern of Human L-Ferritin in Pichia pastoris
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Dietary factors associated with high serum ferritin levels in postmenopausal women with the Fifth Korea National Health and Nutrition Examination Survey (KNHANES V), 2010-2012
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미국흰불나방(Hyphantria cunea) 유충 혈림프의 Ferritin 정제
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The Effects of Visible Light on Iron Release from Ferritin Related to Lipid Peroxidation in the Retina
Journal of photoscience : an international journal officail organ of the korean society of photoscience
2002 .01
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붉은머리재주나방(Phalera minor)의 유충 혈림프로 부터 Ferritin의 정제와 특성
한국곤충학회지
1995 .01
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Severe Hemorrhage Induced Expressions of Ferritin and Heme Oxygenase-1 in Leukocytes
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Association of serum ferritin level and depression with respect to the body mass index in Korean male adults
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Association of serum ferritin level and depression with respect to the body mass index in Korean male adults
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Spin properties of ferritin within PVA nanofibers
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2008 .02
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Properties of ferritin embedded in PVA nanofibers
한국진공학회 학술발표회초록집
2007 .08
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저열량 생즙 식이가 성인의 혈청 페리틴 농도에 미치는 영향
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Cloning and Expression of Galleria mellonella Ferritin That Encodes Two Major Subunits
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