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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제4호
- 발행연도
- 2008.1
- 수록면
- 639 - 647 (9page)
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초록· 키워드
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The heat-inducible expression vectors for Corynebacterium
glutamicum and C. ammoniagenes were constructed by using
the λOL1 and the cryptic promoters, CJ1 and CJ4 that
express genes constitutively in C. ammoniagenes.. Although
the promoters were isolated from C. ammoniagenes, CJ1
and CJ4 were also active in C. glutamicum. To construct
vectors, the OL1 from the λPL promoter was isolated and
fused to the CJ1 and CJ4 promoters by recombinant
PCR. The resulting artificial promoters, CJ1O and CJ4O,
which have one λOL1, and CJ1OX2, which has two
successive λOL1, were fused to the green fluorescent
protein (GFP) gene followed by subcloning into pCES208.
The expression of GFP in the corynebacteria harboring
the vectors was regulated successfully by the temperaturesensitive
cI857 repressor. Among them, C. ammoniagenes
harboring plasmid pCJ1OX2G containing GFP fused to
CJ1OX2 showed more GFP than the other ones and the
expression was tightly regulated by the repressor. To
construct the generally applicable expression vector using
the plasmid pCJ1OX2G, the His-tag, enterokinase (EK)
moiety, and the MCS were inserted in front of the GFP
gene. Using the vector, the expression of pyrR from C.
glutamicum was tried by temperature shift-up. The results
indicated that the constructed vectors (pCeHEMG) can
be successfully used in the expression and regulation of
foreign genes in corynebacteria.
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참고문헌 (21)
참고문헌 신청
Billman-Jacobe, H. / 1995 / Expression and secretion of heterologous proteases by Corynebacterium glutamicum. Appl. Environ / Microbiol 61 : 1610 ~ 1613
Cadenas, R. F. / 1996 / Construction of new cloning vectors for Brevibacterium lactofermentum. FEMS Microbiol / Lett 137 : 63 ~ 68
Cortay, J. C. / 1991 / Regulation of the acetate operon in Escherichia coli: Purification and functional characterization of the IcIR repressor / EMBO 10 : 675 ~ 679
Deb, J. K. / 1999 / Plasmids of corynebacteria. FEMS Microbiol / Lett 175 : 11 ~ 20
Eikmanns, B. J. / 1991 / A family of Corynebacterium glutamicum-Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing / Gene 102 : 93
Cadenas, R. F. / 1996 / Construction of new cloning vectors for Brevibacterium lactofermentum. FEMS Microbiol / Lett 137 : 63 ~ 68
Cortay, J. C. / 1991 / Regulation of the acetate operon in Escherichia coli: Purification and functional characterization of the IcIR repressor / EMBO 10 : 675 ~ 679
Deb, J. K. / 1999 / Plasmids of corynebacteria. FEMS Microbiol / Lett 175 : 11 ~ 20
Eikmanns, B. J. / 1991 / A family of Corynebacterium glutamicum-Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing / Gene 102 : 93
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