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The effects of the exopolymers of Aureobasidiumpullulans SM-2001 containing β-1,3/1,6-glucan on formalin-induced chronic inflammation were observed. Doses of 62.5, 125,and 250 mg/kg of the exopolymers were orally administered oncea day for 10 days to formalin-induced chronic inflamatorymice (0.02 ml of 3.75% formalin was subaponeuroticalyinjected into the left hind paw), and then the bilateral hind-pawthickness and volume were measured daily, while the pawanalyses were conducted at termination. The results werecompared with those for diclofenac, indomethacin, anddexamethasone (intraperitoneally injected) 15 mg/kg-dosedgroups. Al the animals were sacrificed 10 days after dosing.As a result of the formalin injection, a marked increase in thediference between the intact and formalin-induced pawthickness and volume was detected in the formalin-injectedcontrol compared with that in the intact control with time,weights was also dramatically increased. In a histological andhistomorphometrical analysis, severe histological profiles ofchronic inflammation were detected in the formalin-injectedcontrol with a marked increase in the thickness of the skin ofthe dorsum pedis. However, these formalin-induced chronicinflammatory changes were significantly and dose-dependentlydecreased by the exopolymer treatment. In conclusion, theexopolymer treatment inhibited the chronic inflammatory responseinduced by formalin injection in the mice. However, somewhatdiclofenac-, indomethacin-, and dexamethasone-treated groups.

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