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Cell growth and the production of pullulan byAureobasidium pullulan HP-2001, the UV-induced mutant ofA. pullulans ATCC 42023, increased with increased concentrationof glucose up to 15.0% (w/v). Maximal production of pullulanin the flask scale was 27.65 g/l, when concentrations of glucoseand yeast extract were 15.0 and 0.25% (w/v), respectively.Maximal conversion rate of pullulan from glucose as the carbonsource was 0.37, when those of glucose and yeast extractwere 5.0 and 0.15% (w/v), respectively. On the basis of totalamount of pullulan, the conversion rate of pullulan fromglucose, and utilization rate of glucose to cell mass and pullulanby A. pullulans HP-2001, the optimal concentrations of glucoseand yeast extract for the mass production of pullulan weredetermined to be 10.0 and 0.25% (w/v), respectively, at whichconcentrations the production of pullulan and its conversionrate were 27.14 g/l and 0.27, respectively. Maximal productionof pullulan with optimized concentrations of carbon and nitrogensources by A. pullulans HP-2001 in a 7-1 bioreactor was32.12 g/l for 72 h culture, and that in a 100-l bioreactor withthe inner pressure of 0.4 kgf/cm2 was 36.87 g/l. Increased innerpressure of a 100-l bioreactor resulted in a higher concentrationof dissolved oxygen in the medium, which might enhance theproduction of pullulan by A. pullulans HP-2001.

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