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A moderately halophilic, Gram-positive, spore-forming bacterium was isolated from the roots of Blutaparonportulacoides, a plant found in sandy soil parallel to the beachstrain, designated M9T, was motile and strictly aerobic withrod-shaped cells. It grew in the absence of NaCl and up to20% NaCl, and was able to hydrolyze casein and starch. StrainM9T had a cell-wall peptidoglycan based on L-Orn-D-Asp, thepredominant menaquinone present was menaquinone-7 (MK-7),diaminopimelic acid was not found, and anteiso-C15:0 andiso-C15:0 were the major fatty acids. A phylogenetic analysisTbelonged to the genus Halobacilusand exhibited 16S rRNAgene similarity levels of 97.8-99.4% with the type strains ofthe other nine Halobacillus species. The DNA-DNA relatednessof strain M9T with H. trueperi, the closest relative as regards16S rRNA gene similarity, and H. locisalis was 21% and 18%,respectively. Therefore, on the basis of phenotypic, genotypic,and phylogenetic data, strain M9TT, =CIP108771T, =KCTC 3980T) should be placed in the genusHalobacillus as a member of a novel species, for which thename Halobacilus blutaparonensis sp. nov. is proposed.

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