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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제2호
- 발행연도
- 2004.1
- 수록면
- 337 - 343 (7page)
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초록· 키워드
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A bacterial strain concurrently producing extracellularchitosanase and chitinase was isolated from soil and identifiedas a member of the b-subgroup of Proteobacteria through its16S rRNA analysis and some biochemical analyses. Thenewly discovered strain, named as KNU3, had 99% homologyof its 16S rRNA sequence with chitinolytic b-ProteobacteriumCTE108. Strain KNU3 produced 34 kDa of chitosanase inaddition to two chitinases of 68 kDa and 30 kDa, respectively.The purified chitosanase protein (ChoK) showed activitytoward soluble, colloidal, and glycol chitosan, but did notexhibit any activity toward colloidal chitin. The optimum pHand temperature of ChoK were 6.0 and 70oC, respectively.The chitosanase was stable in the pH 4.0 to 8.0 rangeat 70oC, while enzyme activity was relatively stable atbelow 45oC. MALDI-TOF MS and N-terminal amino acidsequence analyses indicated that ChoK protein is related tochitosanases from Matsuebacter sp. and Sphingobacteriummultivorum. HPLC analysis of chitosan lysates revealed thatglucosamine tetramers and hexamers were the major productsof hydrolysis.
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참고문헌 (22)
참고문헌 신청
/ 1990 / Japanase Society for Chitin and Chitosan applicatio (f chitin and chitosan) : 71 ~ 98
Barns / lane1991 / 16S ribosomal DNA amplification for phylogeneticstudy : 697 ~ 703
Fenton / 1981 / Purification andmode of action of a chitosanase from Penicilliumislandicum : 151 ~ 165
Hong / 2003 / Cloning and Characterization of a bifunctional cellulasechitosanasegene from Bacillus licheniformis NBL420 13 : 35 ~ 42
I / 1992 / Action pattern of Bacillus sp 7-Mchitosanase on partially N-acetylated chitosan : 448 ~ 453
Barns / lane1991 / 16S ribosomal DNA amplification for phylogeneticstudy : 697 ~ 703
Fenton / 1981 / Purification andmode of action of a chitosanase from Penicilliumislandicum : 151 ~ 165
Hong / 2003 / Cloning and Characterization of a bifunctional cellulasechitosanasegene from Bacillus licheniformis NBL420 13 : 35 ~ 42
I / 1992 / Action pattern of Bacillus sp 7-Mchitosanase on partially N-acetylated chitosan : 448 ~ 453
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족두리에 관한연구
복식
1999 .03
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Purification and Characterization of Chitinase from a Marine Bacterium, Vibrio sp. 98CJ11027
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