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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제12호
- 발행연도
- 2011.1
- 수록면
- 1,312 - 1,321 (10page)
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Enzyme extracts of cellulase [filter paper cellulase (FPase)and carboxymethyl cellulase (CMCase)], chitinase, and chitosanase produced by Aspergillus niger NRRL-567were evaluated. The interactive effects of initial moisture and different inducers for FP cellulase and CMCase production were optimized using response surface methodology. Higher enzyme activities [FPase 79.24±4.22 IU/gram fermented substrate (gfs) and CMCase 124.04±7.78 IU/gfs] were achieved after 48 h fermentation in solid-state medium containing apple pomace supplemented with rice husk [1% (w/w)] under optimized conditions [pH 4.5, moisture 55% (v/w), and inducers veratryl alcohol (2 mM/kg), copper sulfate (1.5 mM/kg), and lactose 2%(w/w)] (p<0.05). Koji fermentation in trays was carried out and higher enzyme activities (FPase 96.67±4.18 IU/gfs and CMCase 146.50±11.92 IU/gfs) were achieved. The nonspecific chitinase and chitosanase activities of cellulase enzyme extract were analyzed using chitin and chitosan substrates with different physicochemical characteristics,such as degree of deacetylation, molecular weight, and viscosity. Higher chitinase and chitosanase activities of 70.28±3.34 IU/gfs and 60.18±3.82 to 64.20±4.12 IU/gfs,respectively, were achieved. Moreover, the enzyme was stable and retained 92-94% activity even after one month.
Cellulase enzyme extract obtained from A. niger with chitinolytic and chitosanolytic activities could be potentially used for making low-molecular-weight chitin and chitosan oligomers, having promising applications in biomedicine,pharmaceuticals, food, and agricultural industries, and in biocontrol formulations.
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참고문헌 (22)
참고문헌 신청
Alvarez, J. M / 2009 / Expression of genes encoding laccase and manganese-dependent peroxidase in the fungus Ceriporiopsis subvermispora is mediated by an ACE1-like copper-fi
Aro, N. / 2001 / ACEII, a novel transcriptional activator involved in regulation of cellulase and xylanase genes of Trichoderma reesei / J. Biol. Chem 276 : 24309 ~ 24314
Brar, S. K / 2007 / Bacillus thuringiensis fermentation of wastewater and wastewater sludge:Presence and characterization of chitinases / Environ. Technol 29 : 161 ~ 170
Brijwani, K / 2010 / Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran / Proc. Biochem 45 : 1
Cen, P / 1999 / Production of cellulase in solid state fermentation in Recent Progress in Bioconversion of Lignocellulosics. Advances in Biochemical Engineering/Biotechnology, 6.5 / Spri
Aro, N. / 2001 / ACEII, a novel transcriptional activator involved in regulation of cellulase and xylanase genes of Trichoderma reesei / J. Biol. Chem 276 : 24309 ~ 24314
Brar, S. K / 2007 / Bacillus thuringiensis fermentation of wastewater and wastewater sludge:Presence and characterization of chitinases / Environ. Technol 29 : 161 ~ 170
Brijwani, K / 2010 / Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran / Proc. Biochem 45 : 1
Cen, P / 1999 / Production of cellulase in solid state fermentation in Recent Progress in Bioconversion of Lignocellulosics. Advances in Biochemical Engineering/Biotechnology, 6.5 / Spri
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