지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제1호
- 발행연도
- 2007.1
- 수록면
- 116 - 122 (7page)
이용수
초록· 키워드
오류제보하기
An eficient enzyme system for the synthesis ofL-tyrosine was developed using a fed-batch reactor withcontinuous feding of phenol, pyruvate, and amonia. Athermo- and chemostable tyrosine phenol-lyase fromSymbiobacterium toebii was employed as the biocatalyst inexpression system in Escherichia coli BL21, and prepared as asoluble extract by rapid clarification, involving treatment with40% methanol in the presence of excess ammonium chloride.The stability of the enzyme was maintained for at least 18 hunder the synthesis conditions, including 75 mM phenol atpH 8.5 and 40oC. The fed-batch system (working volume,0.5 l) containing 1.0 kU of the enzyme preparation wascontinuously fed with two substrate preparations: one containingcontaining 0.4 mM pyridoxal-5-phosphate and 4 M amoniumchloride (pH 8.5). The system produced 130 g/l of L-tyrosinewithin 30 h, mostly as precipitated particles, upon continuousfeeding of the substrates for 22 h. The maximum conversionyield of L-tyrosine was 94% on the basis of the supplied phenol.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
/ 1979 / 4- dihydroxyphenylalanine synthesis by the bacterium Citrobacter freundii : 827 ~ 831
A / 1951 / The use of organic solvents at low temperature for the separation of enzymes Application to aqueous rabbit muscle extract : 42 ~ 48
A / 1984 / Tyrosine for the treatment of depression : 163 ~ 3 173
Foor / 1993 / Production of L-dihydroxyphenylalanine in Escherichia coli with the tyrosine phenol-lyase gene cloned from Erwinia herbicola : 3070 ~ 3075
Fukui / 1975 / Production of L-tryptophan L-tyrosine and their analogues by use of immobilized tryptophanases and immobilized β-tyrosinase : 25 ~ 39
A / 1951 / The use of organic solvents at low temperature for the separation of enzymes Application to aqueous rabbit muscle extract : 42 ~ 48
A / 1984 / Tyrosine for the treatment of depression : 163 ~ 3 173
Foor / 1993 / Production of L-dihydroxyphenylalanine in Escherichia coli with the tyrosine phenol-lyase gene cloned from Erwinia herbicola : 3070 ~ 3075
Fukui / 1975 / Production of L-tryptophan L-tyrosine and their analogues by use of immobilized tryptophanases and immobilized β-tyrosinase : 25 ~ 39
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Tyrosine Phenol-Lyase의 고속탐색기술 개발 및 방향성 분자진화
한국미생물·생명공학회지
2006 .01
-
Studies on the Microbial Pigment (Ⅲ) : The Effect of Tyrosine
미생물학회지
1978 .03
-
Enhanced Stability of Tyrosine Phenol-Lyase from Symbiobacterium toebiiby DNA Shuffling
Journal of Microbiology and Biotechnology
2004 .01
-
Metabolism of Tyrosine and Leucine during Metamorphosis of Bombyx mori L.
한국곤충학회지
1982 .01
-
Regulation of Cav3.2 Ca Channel Activity by Protein Tyrosine Phosphorylation
Journal of Microbiology and Biotechnology
2008 .01
-
Metabolism of Tyrosine during the Formation and Hardening Process of Cuticle in Pieris rapae L.
한국곤충학회지
1979 .01
-
Identification of a Potential Tyrosine Phosphorylation Site on the NR2B Subunit of the N-methyl-D-aspartate Receptor
생명과학회지
1998 .12
-
Screening of Marine Microbial Extracts for Tyrosine Phosphatase 1B Inhibitors
한국해양바이오학회지
2007 .12
-
Effect of High-tyrosine Diet on Brain Norepinephrine Metabolism in Immobilization-Stressed Rats
한국영양학회지
1993 .10
-
한국산 감자의 Phenol 화합물의 동향에 관한 연구
동아시아식생활학회 학술발표대회논문집
2006 .05
-
Comparison of Adsorption Configurations between Phenylalanine and Tyrosine on Ge(100)
한국진공학회 학술발표회초록집
2011 .08
-
Biological Screening of a Novel Nickel (II) Tyrosine Complex
Mycobiology
2007 .01
-
Tryptophan 및 Tyrosine 보강 식이가 Stress로 인한 혈청 Cortisol, Glucose 및 Free Fatty Acid의 농도 변화에 미치는 영향
한국영양학회지
1990 .08
-
Purity Analysis of L-Tyrosine
한국분석과학회 학술대회
2012 .11
-
Saccharification of Brown Macroalgae Using an Arsenal of Recombinant Alginate Lyases: Potential Application in the Biorefinery Process
Journal of Microbiology and Biotechnology
2018 .01
-
Phenol 분해균주의 분리 및 페놀함유 폐수의 생물학적 처리
생명과학회지
2013 .10
-
Activation of Phospholipase D2 through Phosphorylation of Tyrosine-470 in Antigen-stimulated Mast Cells
생명과학회지
2005 .06
-
The adsorption and photochemical studies of phenol / Ag(111
한국진공학회 학술발표회초록집
2002 .02
-
Alginate Lyase 생산 균주 Pseudomonas sp. N7151-6의 분리 및 특성
한국미생물·생명공학회지
2009 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0