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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 350 - 354 (5page)
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초록· 키워드
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A Gram-negative, alginate lyase-producing bacterium was isolated from the Haeundae Coast, Korea. The isolated strain N7151-6 produced
alginate lyase. The optimal temperature and pH for growth were found to be 30℃ and pH 8.0, respectively.
This strain can be grown at the NaCl concentration of 0-7% (w/v). Analysis of 16S rDNA sequence and physiological profiling indicated that the strain N7151-6 belonged to Pseudomonas sp. The enzyme alginate
lyase produced by Pseudomonas sp. N7151-6 was partially purified by ultrafiltration (MWCO= 30 kDa). The optimum pH and temperature for the activity of the purified enzyme were found to be 7.0 and 30℃, respectively.
The enzyme was stable at the pH range of 5.0-9.0 and temperature range of 23-37℃. The total activity of alginate lyase produced was reached about 110 unit/L.
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참고문헌 (23)
참고문헌 신청
[단행
Anzai, H / 1990 / Determination of D-mannuronic to L-guluronic acids ratio in acid hydrolysis under improved conditions / Nippon Suisan Gakkaishi 56 : 73 ~ 81
Davidson, I. W / 1976 / Purification and properties of an alginate lyase from a marine bacterium / Biochem. J. 159 : 707 ~ 713
Dunne, W. M / 1985 / Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeroguinosa isolated from a pa
Franklin, M. J / 2007 / Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase / J. Bacteriol 186 : 4759 ~ 4773
Anzai, H / 1990 / Determination of D-mannuronic to L-guluronic acids ratio in acid hydrolysis under improved conditions / Nippon Suisan Gakkaishi 56 : 73 ~ 81
Davidson, I. W / 1976 / Purification and properties of an alginate lyase from a marine bacterium / Biochem. J. 159 : 707 ~ 713
Dunne, W. M / 1985 / Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeroguinosa isolated from a pa
Franklin, M. J / 2007 / Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase / J. Bacteriol 186 : 4759 ~ 4773
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