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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제1호
- 발행연도
- 2004.1
- 수록면
- 153 - 157 (5page)
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초록· 키워드
오류제보하기
Tyrosine phenol-lyase (TPL) is a useful enzymefor the synthesis of pharmaceutical aromatic amino acids. Inthe current study, sequential DNA shuffling and screeningwere used to enhance the stability of TPL. Twenty-thousandmutants were screened, and several improved variants wereisolated. One variant named A13V, in which the 13th aminoacid alanine was substituted by valine, exhibited a highertemperature and denaturant stability than the wild-type TPL.The purified mutant TPL, A13V, retained about 60% of itsactivity at 76oC, whereas the activity of the wild-type TPLdecreased to less than 20% at the same temperature. Plus,A13V exhibited about 50% activity with 3 M urea, while thewild-type TPL lost almost all its catalytic activity, indicatingan increased denaturant tolerance in the mutant A13V. It isspeculated that the substitution of Val for the Ala in theb-strand of the N-terminal arm was responsible for theheightened stabilization, and that the current results willcontribute to further research on the structural stability ofTPL.
목차
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참고문헌 (14)
참고문헌 신청
/ 1970 / Thermostability of wild-type TPL See Materials and Methods for details Denaturant tolerance of wild-type TPL See Materials and Methods for details ENHANCED STABILITY
/ 1971 / Enzymatic preparation of L-tyrosine and 3 : 4 1345 ~ 1349
/ 1973 / Synthesis of L-tyrosine and 3 and phenol or pyrocatechol : 4 725 ~ 735
/ 1975 / Synthesis of L-tyrosinerelatedamino acids by b-tyrosinase : 249 ~ 288
/ 1981 / Investigation of the conditions forthe synthesis of tyrosine and 3 : 278 ~ 283
/ 1971 / Enzymatic preparation of L-tyrosine and 3 : 4 1345 ~ 1349
/ 1973 / Synthesis of L-tyrosine and 3 and phenol or pyrocatechol : 4 725 ~ 735
/ 1975 / Synthesis of L-tyrosinerelatedamino acids by b-tyrosinase : 249 ~ 288
/ 1981 / Investigation of the conditions forthe synthesis of tyrosine and 3 : 278 ~ 283
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