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The selection of multienzyme complex-producingbacteria under aerobic condition was conducted for improvingthe degradation of lignocellulosic substances. The criteria forselection were cellulase and xylanase enzyme production, thepresence of celulose-binding domains and/or xylan-bindingadhesion of bacterial cells to insoluble substances, and theproduction of multiple cellulases and xylanases in a form of ahigh molecular weight complex. Among the six Bacilusstrains, isolated from various sources and deposited in ourlaboratory, Paenibacillus curdlanolyticus B-6 strain was thebest producer of cellulase and xylanase enzymes, which haveboth cellulose-binding factors (CBFs) and xylan-binding factors(XBFs). Moreover, multiple carboxymethyl cellulases (CMCases)and xylanases were produced by the strain B-6. The6 types of CMCases associated in a protein band of xylanaseand cellulase with high molecular weight. These cells alsoenabled to adhere to both avicel and insoluble xylan, whichwere analyzed by scanning electron microscopy. The resultsindicated that the strain B-6 produced the multienzymecomplex, which may be cellulosome or xylanosome. Thus, P.curdlanolyticus B-6 was selected to study the role andinteraction betwen the enzymes and their substrates and thecoperation of multiple enzymes to enhance the hydrolysisdue to the complex structure for efficient cellulases and

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