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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제11호
- 발행연도
- 2012.1
- 수록면
- 1,501 - 1,509 (9page)
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A novel bacterial strain, MG7, with high cellulase activity was isolated and identified by morphological characteristics and molecular phylogeny analysis as Paenibacillus barcinonensis. Maximum production of cellulase by MG7was observed at pH 7.0 and 35oC. The enzyme was purified with a specific activity of 16.88 U/mg, the cellulase activity was observed in a zymogram, and its molecular mass (58.6 kDa) was confirmed by SDS-PAGE. The purified enzyme showed maximum activity at pH 6.0 and 65oC and degraded cellulosic substrates such as carboxy methyl cellulose (CMC), Avicel, filter paper, and β-glucan. The enzyme showed stability with 0.5% concentration of various surfactants. The Km and Vmax of cellulase for CMC and Avicel were found to be 0.459mg/ml and 10.46mg/ml/h,and 1.01 mg/ml and 10.0 mg/ml/h, respectively. The high catalytic activity and its stability to temperature, pH,surfactants, and metal ions indicated that the cellulase enzyme by MG7 is a good candidate for biotechnological applications.
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참고문헌 (22)
참고문헌 신청
Adsul, M. G. / 2007 / Strain improvement of Penicillium janthinellum NCIM1171 for increased cellulose production / Bioresour. Technol 98 : 1467 ~ 1473
Ash, C. / 1993 / Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of new genus Paenibac
Bailey, M. J. / 1992 / Interlaboratory testing of methods for assay of xylanase activity / J. Biotechnol 23 : 257 ~ 270
Castanon, M. / 1981 / Effects of the surfactant Tween 80 on enzymatic hydrolysis of newspaper / Biotechnol. Bioeng 23 : 1365 ~ 1372
Choudhary, N. / 1980 / Reducing sugar accumulation from alkali pretreated sugar cane bagasse using Cellulomonas / Eur. J. Appl. Microbiol 11 : 50 ~ 54
Ash, C. / 1993 / Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of new genus Paenibac
Bailey, M. J. / 1992 / Interlaboratory testing of methods for assay of xylanase activity / J. Biotechnol 23 : 257 ~ 270
Castanon, M. / 1981 / Effects of the surfactant Tween 80 on enzymatic hydrolysis of newspaper / Biotechnol. Bioeng 23 : 1365 ~ 1372
Choudhary, N. / 1980 / Reducing sugar accumulation from alkali pretreated sugar cane bagasse using Cellulomonas / Eur. J. Appl. Microbiol 11 : 50 ~ 54
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