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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제3호
- 발행연도
- 2011.1
- 수록면
- 284 - 292 (9page)
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An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan.
The temperature and pH optima for growth were 60oC and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and Avicel. A scanning electron microscopy analysis showed the adhesion of xylan to the cells. An endocellulase-free multienzyme complex was isolated from the crude enzyme of strain NOI-1 by affinity purification on cellulose and Sephacryl S-300 gel filtration.
The molecular mass of the multienzyme complex was estimated to be about 1,200 kDa. The multienzyme complex showed one protein on native PAGE, one xylanase on a native zymogram, 21 proteins on SDS-PAGE, and 5xylanases on a SDS zymogram. The multienzyme complex consisted of xylanase, β-xylosidase, α-L-arabinofuranosidase,β-glucosidase, and cellobiohydrolase. The multienzyme complex was effective in hydrolyzing xylan and corn hulls.
This is the first report of an endocellulase-free multienzyme complex produced by a thermophilic anaerobic bacterium,T. thermosaccharolyticum strain NOI-1.
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참고문헌 (22)
참고문헌 신청
Bachmann, S. L / 1991 / Purification and cooperative activity of enzymes constituting the xylan-degrading system of Thermomonospora fusca / Appl. Environ. Microbiol 57 : 2121
Bayer, E. A / 1986 / Ultrastructure of the cell surface cellulosome of Clostridium thermocellum and its interaction with cellulose / J. Bacteriol 167 : 828 ~ 836
Bayer, E. A / 2004 / The cellulosomes. Multienzyme machines for degradation of plant cell wall polysaccharides / Annu. Rev. Microbiol 58 : 521 ~ 554
Beg, Q. K / 2001 / Microbial xylanases and their industrial applications / Appl. Microbiol. Biotechnol 56 : 326 ~ 338
Biely,P / 1985 / Microbial xylanolytic systems / Trends Biotechnol 3 : 286 ~ 290
Bayer, E. A / 1986 / Ultrastructure of the cell surface cellulosome of Clostridium thermocellum and its interaction with cellulose / J. Bacteriol 167 : 828 ~ 836
Bayer, E. A / 2004 / The cellulosomes. Multienzyme machines for degradation of plant cell wall polysaccharides / Annu. Rev. Microbiol 58 : 521 ~ 554
Beg, Q. K / 2001 / Microbial xylanases and their industrial applications / Appl. Microbiol. Biotechnol 56 : 326 ~ 338
Biely,P / 1985 / Microbial xylanolytic systems / Trends Biotechnol 3 : 286 ~ 290
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