지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 63 - 70 (8page)
이용수
초록· 키워드
오류제보하기
A functional screen of 60,672 fosmid metagenomic clones amplified from marine sediment obtained from the Dokdo islets in Korea identified the gene EstES1, whose product, EstES1, displayed lipolytic properties on tributyrin-supplemented media. EstES1 is a 576 amino acid protein with a predicted molecular weight of 59.4 kDa including 37 N-terminal leader amino acids. EstES1 exhibited the highest sequence similarity (44%) to a carboxylesterase found in Haliangium ochraceum DSM14365. Phylogenetic analysis indicated that EstES1 belongs to a currently uncharacterized family of lipases. Within the conserved domain, EstES1 retains the catalytic triad that consists of the consensus penta-peptide motif, GESAG. EstES1 demonstrated a broad substrate specificity toward the long acyl group of ethyl esters (C2−C12), and its optimal activity was recorded toward p-Nitrophenyl butyrate (C4) at pH 9.0 and 40℃ (specific activity of 255.4 U/mg). The enzyme remained stable in the ranges of 60−65℃ and pH 9.0-10.5 and in the presence of methanol, ethanol, isopropanol, and dimethyl sulfoxide. Therefore, EstES1 has potential for use in industrial applications involving high temperature, organic solvents, and/or alkaline conditions.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Alma'abadi AD / 2015 / Marine metagenome as a resource for novel enzymes / Genomics Proteomics Bioinformatics 13 : 290 ~ 295
Anbu P / 2015 / Microbial enzymes and their applications in industries and medicine 2014 / Biomed. Res. Int 2015 : 816419 ~
Arpigny JL / 1999 / Bacterial lipolytic enzymes: classification and properties / Biochem J 343 (Pt 1) : 177 ~ 183
Bornscheuer UT / 2002 / Microbial carboxyl esterases: classification, properties and application in biocatalysis / FEMS Microbiol. Rev 26 : 73 ~ 81
Chu X / 2008 / Identification of two novel esterases from a marine metagenomic library derived from South China Sea / Appl. Microbiol. Biotechnol 80 : 615 ~ 625
Anbu P / 2015 / Microbial enzymes and their applications in industries and medicine 2014 / Biomed. Res. Int 2015 : 816419 ~
Arpigny JL / 1999 / Bacterial lipolytic enzymes: classification and properties / Biochem J 343 (Pt 1) : 177 ~ 183
Bornscheuer UT / 2002 / Microbial carboxyl esterases: classification, properties and application in biocatalysis / FEMS Microbiol. Rev 26 : 73 ~ 81
Chu X / 2008 / Identification of two novel esterases from a marine metagenomic library derived from South China Sea / Appl. Microbiol. Biotechnol 80 : 615 ~ 625
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A Critical Hydrogen Bond Determines the Catalytic Activity of Hyperthermophilic Esterase EstE1
한국미생물학회 학술대회논문집
2015 .04
-
Characterization of Novel Salt-Tolerant Esterase Isolated from the Marine Bacterium Alteromonas sp. 39-G1
Journal of Microbiology and Biotechnology
2020 .01
-
In Silico Metagenomes Mining to Discover Novel Esterases with Industrial Application by Sequential Search Strategies
Journal of Microbiology and Biotechnology
2015 .01
-
Enhanced Structural Flexibility of an Organic Solvent-Tolerant Lipase by Dimethyl Sulfoxide and Ethanol
한국미생물학회 학술대회논문집
2015 .04
-
Organic Solvent-Tolerant Esterase from Sphingomonas glacialis Based on Amino Acid Composition Analysis: Cloning and Characterization of EstSP2
Journal of Microbiology and Biotechnology
2018 .01
-
Klebsiella pneumoniae의 esterase유전자 cloning과 대장균에서의 고발현
미생물학회지
2021 .03
-
Production of Tween 80-inducing Esterase by Acinetobacter sp. B1 Using Response Surface Methodology
한국미생물·생명공학회지
2019 .01
-
Gene Cloning, High-Level Expression, and Characterization of an Alkaline and Thermostable Lipase from Trichosporon coremiiforme V3
Journal of Microbiology and Biotechnology
2015 .01
-
재조합 대장균의 고농도 배양과 유도조건 최적화를 통한 Bacillus 유래 esterase의 생산
한국미생물·생명공학회지
2017 .06
-
Molecular Cloning, Characterization, and Application of Organic Solvent-Stable and Detergent-Compatible Thermostable Alkaline Protease from Geobacillus thermoglucosidasius SKF4
Journal of Microbiology and Biotechnology
2024 .02
-
Purification and Characterization of an Alkali-Thermostable Lipase from Thermophilic Anoxybacillus flavithermus HBB 134
Journal of Microbiology and Biotechnology
2016 .01
-
Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase
Journal of Microbiology and Biotechnology
2019 .01
-
Identification and Characterization of a New Alkaline SGNH Hydrolase from a Thermophilic Bacterium Bacillus sp. K91
Journal of Microbiology and Biotechnology
2016 .01
-
Identification and Characterization of a Thermostable Enzyme-producing Ruegeria sp. WJ45-6 Isolated from Seawater Sample of Jeju Island, Korea
한국미생물학회 학술대회논문집
2019 .04
-
D-β-Acetylthioisobutyric acid 생산을 위한 내열성 광학선택적 esterase 활성 Klebsiella pneumoniae의 분리
미생물학회지
2019 .06
-
Microbial Community Composition in the Marine Sediments of Jeju Island: Next-Generation Sequencing Surveys
Journal of Microbiology and Biotechnology
2016 .01
-
Molecular Characterization of a Thermophilic and Salt- and Alkaline-Tolerant Xylanase from Planococcus sp. SL4, a Strain Isolated from the Sediment of a Soda Lake
Journal of Microbiology and Biotechnology
2015 .01
-
Potential Yeast from Indonesian Wild Forest Honey Showing Ability to Produce Lipase for Lipid Transesterification
한국미생물·생명공학회지
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0