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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
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- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제2호
- 발행연도
- 2008.1
- 수록면
- 283 - 286 (4page)
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초록· 키워드
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The methylenetetrahydrofolate dehydrogenase/cyclohydrolase (MTHFDC) from the thermoacidophilic archaeon Thermoplasma acidophilum is a 30.6 kDa molecular-mass enzyme that sequentially catalyzes the conversion of formyltetrahydrofolate to methylenetetrahydrofolate, with a preference for NADP as a cofactor, rather than NAD. In order to elucidate the functional and structural features of MTHFDC from archaeons at a molecular level, it was overexpressed in Escherichia coli and crystallized in the presence of its cofactor, NADP, at 295 K using polyethylene glycol (PEG) 4000 as a precipitant. The crystal is a member of the monoclinic space group P21, with the following unit cell parameters: a=66.333 Å, b=52.868 Å, c=86.099 Å, and β= 97.570o, and diffracts to a resolution of at least 2.40 Å at the synchrotron. Assuming a dimer in the crystallographic asymmetric unit, the calculated Matthews parameter (VM) was 2.44 Å3/Da and the solvent content was 49.7%.
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참고문헌 (20)
참고문헌 신청
Allaire, M. / 1998 / The 3-D structure of a folate-dependent dehydrogenase/cyclohydrolase bifunctional enzyme at 1.5 A resolution / Structure 6 : 173 ~ 182
CHOI, JEONG JIN / 2006 / Cloning, Expression, and Characterization of a Hyperalkaline Phosphatasefrom the Thermophilic Bacterium Thermus sp. T351 / Journal of Microbiology an
CHOI, JUN-HO / 2005 / Cloning and Characterization of Cyclohexanol Dehydrogenase Gene fromRhodococcus sp. TK6 / Journal of Microbiology and Biotechnology 15 (6) : 1185 ~ 1196
Collaborative Computational Project / 1994 / The CCP4 suite: Programs for protein crystallography / Acta Crystallogr D50 : 760 ~ 763
D’Ari, L. / 1991 / Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10- methenyltet
CHOI, JEONG JIN / 2006 / Cloning, Expression, and Characterization of a Hyperalkaline Phosphatasefrom the Thermophilic Bacterium Thermus sp. T351 / Journal of Microbiology an
CHOI, JUN-HO / 2005 / Cloning and Characterization of Cyclohexanol Dehydrogenase Gene fromRhodococcus sp. TK6 / Journal of Microbiology and Biotechnology 15 (6) : 1185 ~ 1196
Collaborative Computational Project / 1994 / The CCP4 suite: Programs for protein crystallography / Acta Crystallogr D50 : 760 ~ 763
D’Ari, L. / 1991 / Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10- methenyltet
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