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Batch cultures were carried out to optimize theexo-polysaccharide production by liquid cultures of Pleurotusferulae. Among the various carbon sources, when 5% ofglucose was used, the maximum mycelial growth and exopolysaccharideconcentration reached were 8.78 g/l and 3.59 g/l,respectively. Yeast extract and polypeptone were identified asthe most suitable nitrogen sources. In particular, when amixture of 1% of polypeptone and 0.8% of yeast extractwas used, 9.52 g/l of mycelial growth and 4.09 g/l of exopolysaccharidewere obtained. In the case of mineral sources,K2HPO4 and MgSO4·7H2O were found to be the best mineralsources for mycelial growth and exo-polysaccharide production.Under the optimized culture conditions, the agitation speedand aeration were investigated for mycelial growth and exopolysaccharideproduction in a jar fermentor. The maximummycelial growth and exo-polysaccharide concentration at 1.5 vvmand 200 rpm obtained were 13.2 g/l and 4.95 g/l, respectively,after 10 days of culture, which were 76% and 79% higherthan those of the basal medium. The specific growth rate wasdecreased with the increase of mycelial growth. However,the specific production rate of the exo-polysaccharidewas proportionally increased with the specific growthrate. The proposed model profiles showed good agreementwith the experimental results for the mycelial growth andexo-polysaccharide production. The specific production rateusing the optimized medium was higher than that of basal medium.

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