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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제11호
- 발행연도
- 2009.1
- 수록면
- 1,490 - 1,495 (6page)
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초록· 키워드
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The development of nanotechnology has penetrated the
fields of biology and medicine, resulting in remarkable
applications for tissue regeneration. In order to apply this
technology to tissue engineering, we have developed nanoscaled
3D scaffolds consisting of growth factor-loaded
heparin/poly(l-lysine) nanoparticles (NPs) attached to the
surface of polymeric microspheres via polyionic complex
methods. Growth factor-loaded NPs were simply produced
as polyelectrolyte complexes with diameters of 100-200 nm.
They were then coated onto positively charged poly(lacticco-
glycolic acid) (PLGA) pretreated with polyethyleneimine
to enable cell adhesion, proliferation, and stimulation of
neurite outgrowth. Propidium iodide staining and β-tubulin
analysis revealed that neuronal PC12 cells proliferated
extensively, expressed significant amounts of b-tubulin,
and showed well-structured neurite outgrowth on polymeric
microspheres by stimulation with growth factors. These
results suggest that cellular adhesion and biological
functionality on prepared PLGA microspheres enabled
terminal differentiation of neuronal cells.
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참고문헌 (23)
참고문헌 신청
Bozkurt, A. / 2007 / In vitro assessment of axonal growth using dorsal root ganglia explants in a novel three-dimensional collagen matrix / Tissue Eng 13 : 2971 ~ 2979
Chapin, J. K. / 2004 / Using multi-neuron population recordings for neural prosthetics / Nat. Neurosci 7 : 452 ~ 455
Claude, P. / 1988 / Basic FGF induces neuronal differentiation, cell division, and NGF dependence in chromaffin cells: A sequence of events in sympathetic debelopment / Neuro
Crompton, K. E. / 2007 / Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering / Biometerials 28 : 441 ~ 449
Dodla, M. C. / 2008 / Differences between the effect of anisotropic and isotropic laminin and nerve growth factor presenting scaffolds on nerve regeneration across long perip
Chapin, J. K. / 2004 / Using multi-neuron population recordings for neural prosthetics / Nat. Neurosci 7 : 452 ~ 455
Claude, P. / 1988 / Basic FGF induces neuronal differentiation, cell division, and NGF dependence in chromaffin cells: A sequence of events in sympathetic debelopment / Neuro
Crompton, K. E. / 2007 / Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering / Biometerials 28 : 441 ~ 449
Dodla, M. C. / 2008 / Differences between the effect of anisotropic and isotropic laminin and nerve growth factor presenting scaffolds on nerve regeneration across long perip
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O-GlcNAc Modification Is Involved in Neurite Outgrowth of Dopaminergic Neuronal Cells.
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Regeneration of a Cartilage Tissue by In Vitro Culture of Chondrocytes on PLGA Microspheres
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The Effect of KCI-Induced Depolarization on Neurite Outgrowth and Neuronal Survival in the Cultured Drosophila CNS Neuron
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p190RhoGAP and Rap-dependent RhoGAP (ARAP3) inactivate RhoA in response to nerve growth factor leading to neurite outgrowth from PC12 cells
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Polysaccharide for protein stability loaded in PLGA microcapsule
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Dexamethasone, a Synthetic Glucocorticoid, Inhibits PDGF-induced Neurite Outgrowth of Hippocampal Progenitor Neuronal Cell Line, HiB5
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LPCLysophosphatidylcholine suppresses apoptosis and induces neurite outgrowth in PC12 cells through activation of phospholipase D2
Experimental and Molecular Medicine
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Poly lactic-co-glycolic acid (PLGA) nanofibrous scaffold chemically coated with Gold-nanoparticles (GNPs) as osteoinductive agent for osteogenesis
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Secretin induces neurite outgrowth of PC12 through cAMP-mitogen-activated protein kinase pathway
Experimental and Molecular Medicine
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Butyrate-induced differentiation of PC12 cells to chromaffin cells involves cell adhesion and induction of extracellular proteins and cell adhesion proteins
Animal cells and systems
2010 .01
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Rx for Tissue Restoration: Regenerative Biology and Medicine
Korean journal of biological sciences
2001 .01
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Phosphatidylinositol 4-phosphate 5-kinase a negatively regulates nerve growth factor-induced neurite outgrowth in PC12 cells
Experimental and Molecular Medicine
2013 .01
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Distinct Effect of Neurotrophins Delivered Simultaneously by an AdenoviralVector on Neurite Outgrowth of Neural Precursor Cells from DifferentRegions of the Brain
Journal of Microbiology and Biotechnology
2007 .01
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NUMERICAL ANALYSIS OF CELL GROWTH IN SCAFFOLDS
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Transcriptional Profiling in Neuronal Differentiation of Stem Cells
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In situ tissue regeneration through host stem cell recruitment
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Cell Adhesion and Growth on Nanostructured Surface
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