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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제7호
- 발행연도
- 2007.1
- 수록면
- 1,106 - 1,112 (7page)
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This report provides the complete nucleotidesequences of the full-length cDNA encoding squalene synthase(SQS) and its genomic DNA sequence from a triterpene-producingfungus, Ganoderma lucidum. The cDNA of the squalenesynthase (SQS) (GenBank Accesion Number: DQ494674)was found to contain an open reading frame (ORF) of 1,404 bpencoding a 468-amino-acid polypeptide, whereas the SQS genomicDNA sequence (GenBank Acesion Number: DQ494675)consisted of 1,984 bp and contained four exons and threG. lucidumgenome. The deduced amino acid sequence of Ganodermalucidum squalene synthase (Gl-SQS) exhibited a high homologywith other fungal squalene synthase genes and contained sixconserved domains. A phylogenetic analysis revealed that G.lucidum SQS belonged to the fungi SQS group, and was moreclosely related to the SQS of U. maydis than to those of otherfungi. A gene expression analysis showed that the expressionincreased after 14 to 20 days of incubation, and reached arelatively high level in the mushroom primordia. Functionalcomplementation of Gl-SQS in a SQS-deficient strain ofSaccharomyces cerevisiae confirmed that the cloned cDNAencoded a squalene synthase.
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참고문헌 (28)
참고문헌 신청
A / 1984 / Ribosomal DNA spacer-length polymorphisms in barley : 8014 ~ 81 8018
A / 1986 / Angiotensin converting enzyme-inhibitory triterpenes from Ganoderma lucidum : 3025 ~ 3028
Chehab, E. W / 2006 / Rice hydroperoxide lyases with unique expression patterns generate distinct aldehyde signatures in Arabidopsis 141 : 121 ~ 134
Cheng / 1990 / Research progress on the chemical component of Ganoderma lucidum triterpenoid : 940 ~ 953
Chin / 1992 / Triterpenoid natural products in the fungus Ganoderma lucidum : 669 ~ 674
A / 1986 / Angiotensin converting enzyme-inhibitory triterpenes from Ganoderma lucidum : 3025 ~ 3028
Chehab, E. W / 2006 / Rice hydroperoxide lyases with unique expression patterns generate distinct aldehyde signatures in Arabidopsis 141 : 121 ~ 134
Cheng / 1990 / Research progress on the chemical component of Ganoderma lucidum triterpenoid : 940 ~ 953
Chin / 1992 / Triterpenoid natural products in the fungus Ganoderma lucidum : 669 ~ 674
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