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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 145 - 153 (9page)
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초록· 키워드
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Among the carotenoid biosynthesis genes, crtI gene encodes the phytoene desaturase (CrtI) enzyme, and phytoene desaturase convert phytoene to lycopene. Phytoene desaturase is involved in the dehydrogenation reaction, in which four single bonds in the phytoene are introduced into a double bond, eliminating eight hydrogen atoms in the process. Phytoene desaturase is one of the key regulating enzyme in carotenoid biosynthetic pathway of various carotenoid biosynthetic organisms. The crtI gene in genomic DNA of Paracoccus haeundaensis was amplified and cloned into a T-vector to analyze the nucleotide sequence. As a result, the crtI gene coding for phytoene desaturase from P. haeundaensis consists of 1,503 base pairs encoding 501 amino acids residues. An expression plasmid containing the crtI gene was constructed, and Escherichia coli cells containing this plasmid produced the recombinant protein of approximately 55 kDa, equivalent to the molecular weight of phytoene desaturase. The expressed protein in cell lysate showed enzymatic activity similar to phytoene desaturase. Phytoene and lycopene were analyzed by HPLC and measured at wavelength of 280 nm and 470 nm, respectively. The Km values for phytoene and NADPH were 11.1 μM and 129.3 μM, respectively.
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참고문헌 (32)
참고문헌 신청
Linnewiel-Hermoni K / 2015 / The anti-cancer effects of carotenoids and other phytonutrients resides in their combined activity / Arch. Biochem. Biophys. 572:28~35
Stahl W / 2003 / Antioxidant activity of carotenoids / Mol. Aspects Med. 24:345~351
Yokoyama A / 1994 / Production of astaxanthin and 4-ketozeaxanthin by the marine bacterium, Agrobacterium aurantiacum / Biosci. Biotechnol. Biochem. 58:1842~1844
Bubrick P / 1991 / Production of astaxanthin from Haematococcus / Bioresour. Technol. 38:237~239
Ludmila T / 2005 / Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. nov / Int. J. Syst. Evol. Microbiol. 55:139~142
Stahl W / 2003 / Antioxidant activity of carotenoids / Mol. Aspects Med. 24:345~351
Yokoyama A / 1994 / Production of astaxanthin and 4-ketozeaxanthin by the marine bacterium, Agrobacterium aurantiacum / Biosci. Biotechnol. Biochem. 58:1842~1844
Bubrick P / 1991 / Production of astaxanthin from Haematococcus / Bioresour. Technol. 38:237~239
Ludmila T / 2005 / Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. nov / Int. J. Syst. Evol. Microbiol. 55:139~142
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