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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 285 - 292 (8page)
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초록· 키워드
오류제보하기
Phytoene desaturase (PDS) is an essential constituent of several carotenoid biosynthetic enzymes, which catalyzes phytoene into f-carotenoids. Here, a cDNA encoding PDS gene named UpPDS in Ulva pertusa was cloned by the approaches of expressed sequence tag sequencing of suppression subtractive hybridization cDNA library and rapid amplification of cDNA ends technique. The results showed that the full-length cDNA of UpPDS was 2029 bp, containing 1599 bp open reading frame and encoding 532 amino acids. Homology analysis showed that the deduced UpPDS protein was highly homologous to the PDS proteins from some algal species. Phylogenetic analysis indicated that the UpPDS was clustered together with PDS of Ectocarpus siliculosus firstly and was more close to PDS proteins from Chlamydomonas reinhardtii and Volvox carteri. The RT-qPCR analysis indicated that the transcript level of UpPDS was upregulated during the period of emersion.
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참고문헌 (28)
참고문헌 신청
Arnold K / 2006 / The SWISSMODEL workspace: a web-based environment for protein structure homology modelling / Bioinformatics 22:195~201
Botella-Pavia P / 2004 / Regulation of carotenoid biosynthesis in plants: evidence for a key role of hydroxymethylbutenyl diphosphate reductase in controlling the supply of plastidial isoprenoid precursors / Plant J 40:188~199
Chang Z / 1996 / Mycobacterium tuberculosis 16-kDa antigen (Hsp16.3) functions as an oligomeric structure in vitro to suppress thermal aggregation / J Biol Chem 271:7218~7223
Cong L / 2010 / cDNA cloning and expression analysis of wheat (Triticum aestivum L.) phytoene and zeta-carotene desaturase genes / Mol Biol Rep 37:3351~3361
Del Campo JA / 2004 / Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta) / Appl Microbiol Biotechnol 64:848~854
Botella-Pavia P / 2004 / Regulation of carotenoid biosynthesis in plants: evidence for a key role of hydroxymethylbutenyl diphosphate reductase in controlling the supply of plastidial isoprenoid precursors / Plant J 40:188~199
Chang Z / 1996 / Mycobacterium tuberculosis 16-kDa antigen (Hsp16.3) functions as an oligomeric structure in vitro to suppress thermal aggregation / J Biol Chem 271:7218~7223
Cong L / 2010 / cDNA cloning and expression analysis of wheat (Triticum aestivum L.) phytoene and zeta-carotene desaturase genes / Mol Biol Rep 37:3351~3361
Del Campo JA / 2004 / Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta) / Appl Microbiol Biotechnol 64:848~854
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