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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제6호
- 발행연도
- 2005.1
- 수록면
- 1,408 - 1,413 (6page)
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초록· 키워드
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Elevated expression of carcinoembryonic antigen(CEA) has been implicated in various biological aspects ofneoplasia such as tumor cell adhesion, metastasis, blocking ofcellular immune mechanisms, and antiapoptosis function. Thus,the CEA could be an important target for anticancer therapy.In this study, we developed Tetrahymena group 1 intronbasedtrans-splicing ribozymes that can specifically targetand replace CEA RNA. To this end, we first determined whichregions of the CEA RNA were accessible to ribozymes byemploying an RNA mapping strategy that was based on atrans-splicing ribozyme library. Next, we assessed the ribozymeactivities by comparing the trans-splicing activities of severalribozymes that targeted different regions of the CEA RNA,and then the ribozyme that could target the most accessiblesite was observed to be the most active with high fidelityin vitro. Moreover, the specific trans-splicing ribozyme wasfound to react with and altered the target CEA transcriptsin mammalian cells with high fidelity. These results suggestthat the Tetrahymena ribozyme can be utilized to replaceCEA RNAs in tumors with a new RNA-harboringanticancer activity, thereby hopefully reverting the malignantphenotype.
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참고문헌 (26)
참고문헌 신청
/ 1976 / Carcinoembryonic antigen in histopathology Immunoperoxidasestaining of conventional tissue sections : 11 ~ 22
/ 1989 / Cell adhesion activity of non-TARGETED TRANS-SPLICING OF CEA RNA 1413specific cross-reacting antigen expressed on CHO cell surface 1164 (embryonicantigen) : 39 ~ 45
/ 1989 / Improvedpredictions of secondary structures for RNA : 7706 ~ 86 7710
/ 1989 / functions as an intercellular adhesionmolecule carci (embryonic antigen) : 327 ~ 334
/ 1992 / Targeted therapy of athymic mice bearingGW-39 human colonic cancer micrometastases with 131Ilabeledmonoclonal antibodies : 6036 ~ 6044
/ 1989 / Cell adhesion activity of non-TARGETED TRANS-SPLICING OF CEA RNA 1413specific cross-reacting antigen expressed on CHO cell surface 1164 (embryonicantigen) : 39 ~ 45
/ 1989 / Improvedpredictions of secondary structures for RNA : 7706 ~ 86 7710
/ 1989 / functions as an intercellular adhesionmolecule carci (embryonic antigen) : 327 ~ 334
/ 1992 / Targeted therapy of athymic mice bearingGW-39 human colonic cancer micrometastases with 131Ilabeledmonoclonal antibodies : 6036 ~ 6044
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