지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제9호
- 발행연도
- 2009.1
- 수록면
- 1,070 - 1,076 (7page)
이용수
초록· 키워드
오류제보하기
Telomerase reverse transcriptase (TERT), which prolongs
the replicative life span of cells, is highly upregulated in
85-90% of human cancers, whereas most normal somatic
tissues in humans express limited levels of the telomerase
activity. Therefore, TERT has been a potential target for
anticancer therapy. Recently, we described a new approach
to human cancer gene therapy, which is based on the
group I intron of Tetrahymena thermophila. This ribozyme
can specifically mediate RNA replacement of human
TERT (hTERT) transcript with a new transcript harboring
anticancer activity through a trans-splicing reaction, resulting
in selective regression of hTERT-positive cancer cells.
However, to validate the therapeutic potential of the
ribozyme in animal models, ribozymes targeting inherent
transcripts of the animal should be developed. In this
study, we developed a Tetrahymena-based trans-splicing
ribozyme that can specifically target and replace the
mouse TERT (mTERT) RNA. This ribozyme can trigger
transgene activity not only also in mTERT-expressing cells
but hTERT-positive cancer cells. Importantly, the ribozyme
could selectively induce activity of the suicide gene, a
herpes simplex virus thymidine kinase gene, in cancer
cells expressing the TERT RNA and thereby specifically
hamper the survival of these cells when treated with
ganciclovir. The mTERT-targeting ribozyme will be useful
for evaluation of the RNA replacement approach as a
cancer gene therapeutic tool in the mouse model with
syngeneic tumors.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Bodnar, A. G. / 1998 / Extension of life-span by introduction of telomerase into normal human cells / Science 279 : 349 ~ 352
Byun, J. / 2003 / Efficient and specific repair of sickle beta-globin RNA by trans-splicing ribozymes / RNA 9 : 1254 ~ 1263
Fiammengo, R. / 2005 / Nucleic acid enzymes / Curr. Opin. Biotechnol 16 : 614 ~ 621
Forsyth, N. R. / 2002 / Telomerase and differentiation in multicellular organisms: Turn it off, turn it on, and turn it off again / Differentiation 69 : 188 ~ 197
Greenberg, R. A. / 1998 / Expression of mouse telomerase reverse transcriptase during development, differentiation and proliferation / Oncogene 16 : 1723 ~ 1730
Byun, J. / 2003 / Efficient and specific repair of sickle beta-globin RNA by trans-splicing ribozymes / RNA 9 : 1254 ~ 1263
Fiammengo, R. / 2005 / Nucleic acid enzymes / Curr. Opin. Biotechnol 16 : 614 ~ 621
Forsyth, N. R. / 2002 / Telomerase and differentiation in multicellular organisms: Turn it off, turn it on, and turn it off again / Differentiation 69 : 188 ~ 197
Greenberg, R. A. / 1998 / Expression of mouse telomerase reverse transcriptase during development, differentiation and proliferation / Oncogene 16 : 1723 ~ 1730
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Specificity of Intracellular Trans-Splicing Reaction by hTERT-Targeting Group I Intron
Genomics & informatics
2005 .01
-
Gene expression responses in vivo by human telomerasereverse transcriptase (hTERT)-targeting trans-splicingribozyme
Experimental and Molecular Medicine
2007 .01
-
An RNA Mapping Strategy to Identify Ribozyme-Accessible Sites on the Catalytic Subunit of Mouse Telomerase
Genomics & informatics
2007 .01
-
Re-Engineering of Carcinoembryonic Antigen RNA with the GroupIntron of Tetrahymena thermophila by Targeted Trans-Splicing
Journal of Microbiology and Biotechnology
2005 .01
-
Tetrahymena thermophila의 group Ⅰ intron에 의한 trans-splicing 반응에 미치는 표적 RNA 구조의 영향분석
미생물학회지
1999 .09
-
In Vivo Target RNA Specificity of Trans-Splicing Phenomena by the Group I Intron
Genomics & informatics
2008 .01
-
Cancer-Specific Induction of Adenoviral E1A Expression by Group I Intron-Based Trans-Splicing Ribozyme
Journal of Microbiology and Biotechnology
2012 .01
-
RNA Mapping of Mutant Myotonic Dystrophy Protein Kinase 3'-Untranslated Region Transcripts
Genomics & informatics
2009 .01
-
Functional Modification of a Specific RNA with Targeted Trans-Splicing
Genomics & informatics
2004 .01
-
RNA cleavage activity of DNA enzyme and Hammerhead ribozyme targeted to TEL/AML1 fusion gene
한국생명과학회 심포지움
2005 .04
-
Hammerhead Ribozymes Reduce the Expression of HBV X gene in Mammalian Cells
한국동물학회 학술대회
1997 .01
-
한탄바이러스 S Genome Segment에 대한 Ribozyme의 제조 및 항바이러스제제로서의 기능조사
The Journal of the Korean Society for Microbiology
1998 .04
-
A Clue for Prebiotic Era : Cross - Catalytic Replication of an RNA Ligase Ribozyme
한국생명과학회 심포지움
2004 .10
-
Replacement of Thymidine Phosphorylase RNA with Group I Intron of Tetrahymena thermophila by Targeted Trans-Splicing
The Journal of Microbiology
2003 .12
-
Comparative Analysis of Intracellular Trans-Splicing Ribozyme Activity Against Hepatitis C Virus Internal Ribosome Entry Site
The Journal of Microbiology
2004 .12
-
Pathogenic diversity of RNA variants and RNA variation-associated factors in cancer development
Experimental and Molecular Medicine
2020 .01
-
C형 간염바이러스(HCV)의 NS5B RNA Replicase에 의해 그 활성이 조절되는 HCV 지놈 표적 Hammerhead 리보자임 개발
미생물학회지
2007 .09
-
Human telomerase catalytic subunit (hTERT) suppresses p53-mediated anti-apoptotic response via induction of basic fibroblast growth factor
Experimental and Molecular Medicine
2010 .01
-
Construction of an RNase P Ribozyme Library System for Functional Genomics Applications
Genomics & informatics
2007 .01
-
C형 간염바이러스(HCV)의 NS5B RNA Replicase에 의해 활성이 유도되는 Hammerhead 리보자임에 의한 HCV 복제 억제 연구
미생물학회지
2011 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0