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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 405 - 407 (3page)
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초록· 키워드
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A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli - Corynebacterium lactofermentum shuttle vector
pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary
at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract.The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of
LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the
mannanase produced by the parental strain, B. subtilis WL-3.
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등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
Adham, S. A / 2003 / Improved vectors for transcriptional/translational signal screening in corynebacteria using the melC operon from Streptomyces glaucescens as reporter / A
Bischoff, K. M / 1361 / Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361 / Proc. Biochem 42 : 1150 ~ 1154
Deb, J. K / 1476 / Intergeneric protoplast fusion between xylanase producing Bacillus subtilis LYT and Corynebacterium acetoacidophilum ATCC 21476 / FEMS Microbiol. Lett. 59
Ki-Hong Yoon / 2008 / Characterization of the Bacillus subtilis WL-3 Mannanase from a Recombinant Escherichia coli / The Journal of Microbiology 46 (3) : 344 ~ 349
Lee, K. -N / 1995 / Construction and transformation of an endogenous plasmid pBL1-free Brevibacterium lactofermentum / Kor. J. Appl. Microbiol. Biotechnol 23 : 164 ~ 169
Bischoff, K. M / 1361 / Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361 / Proc. Biochem 42 : 1150 ~ 1154
Deb, J. K / 1476 / Intergeneric protoplast fusion between xylanase producing Bacillus subtilis LYT and Corynebacterium acetoacidophilum ATCC 21476 / FEMS Microbiol. Lett. 59
Ki-Hong Yoon / 2008 / Characterization of the Bacillus subtilis WL-3 Mannanase from a Recombinant Escherichia coli / The Journal of Microbiology 46 (3) : 344 ~ 349
Lee, K. -N / 1995 / Construction and transformation of an endogenous plasmid pBL1-free Brevibacterium lactofermentum / Kor. J. Appl. Microbiol. Biotechnol 23 : 164 ~ 169
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