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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 559 - 566 (8page)
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Amyloid β (Aβ) neurotoxicity is believed to play a critical role in the pathogenesis of Alzheimer's disease (AD) mainly because of its deposition in AD brain and its neuronal toxicity. However, there have been discrepancies in Aβ-induced cytotoxicity studies, depending on the assay methods. Com-β42-induced in vitro cyto-toxicity might be useful to elucidate the etiological role of Aβ in the pathogenesis of AD. In this study, MTT, CCK-8, calcein-AM/EthD-1 assays as well as thorough microscopic examinations were compara-tively performed after Aβ42 treatment in a neuronal precursor cells (NT2) and a somatic cells (EcR293). Extensive formation of vacuoles was observed at the very early stage of Aβ42 treatment in both cells. Early observation of Aβ42 toxicity as seen in vacuole formation was also shown in MTT assay, but not in CCK-8 and calcein-AM/EthD-1 assays. In addition, Aβ42 treatment dramatically accelerated MTT for-mazan exocytosis, implying its effect on the ex-tensive formation of cytoplasmic vacuoles. Aβ42 seems to cause indirect inhibition on the intracellular MTT reduction as well as vacuole formation and exocytosis enhancement. Following the acute cellular dysfunction induced by Aβ42, the prolonged treatment of micromolar concentration of Aβ42 resulted in slight inhibition on redox and esterase activity. The early Aβ42-induced vacuolated mor-phology and later chronic cytotoxic effect in neuronal cell might be linked to the chronic neurode-generation caused by the accumulation of Aβ42 in AD patients' brain.
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참고문헌 (17)
참고문헌 신청
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/ / Hong ST. Selective neuronal degenera-tion induced by soluble oligomeric amyloid beta protein. FASEB J 2003a 17 : 118 ~ 20
/ / Kim YS. Identification of amyloid beta-peptide responsive genes by cDNA microarray technology involvement of RTP801 in amyloid beta-peptide toxicity. Exp Mol Med 2003b :
/ / Michikawa M. Amyloid beta-protein affects cholesterol metabolism in cultured neurons implications for pivotal role of cholesterol in the amyloid cascade. J Neurosci Res 2
/ / Oh SH. Activation of calcium signaling by hepatitis B virus-X protein in liver cells. Exp Mol Med 2003 : 301 ~ 9
/ / Hong ST. Selective neuronal degenera-tion induced by soluble oligomeric amyloid beta protein. FASEB J 2003a 17 : 118 ~ 20
/ / Kim YS. Identification of amyloid beta-peptide responsive genes by cDNA microarray technology involvement of RTP801 in amyloid beta-peptide toxicity. Exp Mol Med 2003b :
/ / Michikawa M. Amyloid beta-protein affects cholesterol metabolism in cultured neurons implications for pivotal role of cholesterol in the amyloid cascade. J Neurosci Res 2
/ / Oh SH. Activation of calcium signaling by hepatitis B virus-X protein in liver cells. Exp Mol Med 2003 : 301 ~ 9
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